ArticleThe Journal of cell biology1992
Direct proof that the primary site of action of cytochalasin on cell motility processes is actin.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 89 citations in OpenAlex.
- IMA fungus · 2025Review
- Cytochalasans and Their Impact on Actin Filament Remodeling.Biomolecules · 2023Review
- Transcriptome changes underlie alterations in behavioral traits in different types of chicken.Journal of animal science · 2020Article
- Article
- Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity.Biosensors & bioelectronics · 2009Article
- Detecting effects of low levels of cytochalasin B in 3T3 fibroblast cultures by analysis of electrical noise obtained from cellular micromotion.Biosensors & bioelectronics · 2009Article
- Establishment of latrunculin-A resistance in HeLa cells by expression of R183A D184A mutant beta-actin.Oncogene · 2003Article
- Glutamate induces rapid upregulation of astrocyte glutamate transport and cell-surface expression of GLAST.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Cytochalasin D and latrunculin affect chromosome behaviour during meiosis in crane-fly spermatocytes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1998Article
- Involvement of actin microfilaments in the replication of human parainfluenza virus type 3.Journal of virology · 1998Article
- Moderate concentrations of ethanol inhibit endocytosis of the yeast maltose transporter.Applied and environmental microbiology · 1997Article
- Activation of phospholipase D is an early event in integrin-mediated signalling leading to phagocytosis in human neutrophils.Inflammation · 1996Article
- Serum protects against azurophil granule dependent down-regulation of complement receptor type 1 (CR1) on human neutrophils.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 1995Article
- Article
- Article
- Polarized distribution of actin isoforms in gastric parietal cells.Molecular biology of the cell · 1995Article
- Indirect association of ezrin with F-actin: isoform specificity and calcium sensitivity.The Journal of cell biology · 1995Article
- Molecular cloning, expression, and mapping of the high affinity actin-capping domain of chicken cardiac tensin.The Journal of cell biology · 1995Article
- Article
- Transformation of actin-encapsulating liposomes induced by cytochalasin D.Biophysical journal · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously described the isolation of a mutant KB cell (Cyt 1 mutant) resistant to the cytotoxic effect of cytochalasin B (CB). The Cyt 1 mutant carries an altered form of beta-actin (beta'-actin) and lacks normal beta-actin (Toyama, S., and S. Toyama. 1984. Cell. 37:609-614). Increased resistance of the Cyt 1 mutant to CB in vivo is reflected in altered properties of beta'-actin in vitro (Toyama, S., and S. Toyama. 1988. J. Cell Biol. 107:1499-1504). Here, we show that the mutation in beta-actin is solely responsible for the cytochalasin-resistant phenotype of the Cyt mutant. We have isolated a cDNA clone encoding beta'-actin from Cyt 1 cells. Sequence analysis reveals two mutations in the coding region that substitute two amino acid residues (Val139----Met and Ala295----Asp). Expression of the beta'-actin cDNA confers cytochalasin resistance upon transformed cytochalasin-sensitive KB cells. Levels of resistance to CB in the transformed cell clones correlate well with amounts of beta'-actin polypeptide. Both of the two mutations in beta'-actin are necessary for the high level expression of cytochalasin resistance. Overall, we conclude that the primary site of action of cytochalasin on cell motility processes in vivo is actin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.