ArticleMolecular and cellular biology2007
The nucleosome assembly activity of NAP1 is enhanced by Alien.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
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- The Evolution of COP9 Signalosome in Unicellular and Multicellular Organisms.Genome biology and evolution · 2016Article
- Relationship between Structure and Conformational Change of the Vitamin D Receptor Ligand Binding Domain in 1α,25-Dihydroxyvitamin D3 Signaling.Molecules (Basel, Switzerland) · 2015Review
- The CSN/COP9 signalosome regulates synaptonemal complex assembly during meiotic prophase I of Caenorhabditis elegans.PLoS genetics · 2014Article
- NSBP-1 mediates the effects of cholesterol on insulin/IGF-1 signaling in Caenorhabditis elegans.Cellular and molecular life sciences : CMLS · 2013Article
- Regulation of nucleolar chromatin by B23/nucleophosmin jointly depends upon its RNA binding activity and transcription factor UBF.Molecular and cellular biology · 2010Article
- Retinal proteomic changes under different ischemic conditions - implication of an epigenetic regulatory mechanism.International journal of physiology, pathophysiology and pharmacology · 2010Article
- Modular insulators: genome wide search for composite CTCF/thyroid hormone receptor binding sites.PloS one · 2010Article
- Revisiting the COP9 signalosome as a transcriptional regulator.EMBO reports · 2009Review
- Molecular functions of the histone acetyltransferase chaperone complex Rtt109-Vps75.Nature structural & molecular biology · 2008Article
- Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro.Nucleic acids research · 2008Article
- The coregulator Alien.Nuclear receptor signaling · 2007Review
- The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning.The EMBO journal · 2007Article
- CTCF genomic binding sites in Drosophila and the organisation of the bithorax complex.PLoS genetics · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of nucleosomes into chromatin is essential for the compaction of DNA and inactivation of the DNA template to modulate and repress gene expression. The nucleosome assembly protein 1, NAP1, assembles nucleosomes independent of DNA synthesis and was shown to enhance coactivator-mediated gene expression, suggesting a role for NAP1 in transcriptional regulation. Here, we show that Alien, known to harbor characteristics of a corepressor of nuclear hormone receptors such as of the vitamin D receptor (VDR), binds in vivo and in vitro to NAP1 and modulates its activity by enhancing NAP1-mediated nucleosome assembly on DNA. Furthermore, Alien reduces the accessibility of the histones H3 and H4 for NAP1-promoted assembly reaction. This indicates that Alien sustains and reinforces the formation of nucleosomes. Employing deletion mutants of Alien suggests that different regions of Alien are involved in enhancement of NAP1-mediated nucleosome assembly and in inhibiting the accessibility of the histones H3 and H4. In addition, we provide evidence that Alien is associated with chromatin and with micrococcus nuclease-prepared nucleosome fractions and interacts with the histones H3 and H4. Furthermore, chromatin immunoprecipitation and reimmunoprecipitation experiments suggest that NAP1 and Alien localize to the endogenous CYP24 promoter in vivo, a VDR target gene. Based on these findings, we present here a novel pathway linking corepressor function with nucleosome assembly activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.