ArticleThe Plant cell2007
The N-terminal double-stranded RNA binding domains of Arabidopsis HYPONASTIC LEAVES1 are sufficient for pre-microRNA processing.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Arsenic Resistance Protein BcArs2 Modulates Endogenous ROS Homeostasis in Infection Cushions to Enhance the Virulence of Botrytis cinerea Toward Host Plants.Molecular plant pathology · 2026Article
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Article
- Genome-wide profiling of rice Double-stranded RNA-Binding Protein 1-associated RNAs by targeted RNA editing.Plant physiology · 2023Article
- Plant non-coding RNAs function in pollen development and male sterility.Frontiers in plant science · 2023Review
- microRNA production inFrontiers in plant science · 2023Review
- Dynamic Phosphorylation of miRNA Biogenesis Factor HYL1 by MPK3 Involving Nuclear-Cytoplasmic Shuttling and Protein Stability in Arabidopsis.International journal of molecular sciences · 2022Article
- Cytoplasmic HYL1 modulates miRNA-mediated translational repression.The Plant cell · 2021Article
- Divergence of three BRX homoeologs in Brassica rapa and its effect on leaf morphology.Horticulture research · 2021Article
- Irregular adaxial-abaxial polarity rearrangement contributes to the monosymmetric-to-asymmetric transformation ofAoB PLANTS · 2020Article
- A conserved sequence signature is essential for robust plant miRNA biogenesis.Nucleic acids research · 2020Article
- BcpLH organizes a specific subset of microRNAs to form a leafy head in Chinese cabbage (Horticulture research · 2020Article
- MicroRNA166 MonitorsPlant physiology · 2019Article
- Plant microRNAs: Biogenesis, Homeostasis, and Degradation.Frontiers in plant science · 2019Review
- Association of microRNAs with Types of Leaf Curvature inFrontiers in plant science · 2018Article
- Increased Affinity for RNA Targets Evolved Early in Animal and Plant Dicer Lineages through Different Structural Mechanisms.Molecular biology and evolution · 2017Article
- The 'how' and 'where' of plant microRNAs.The New phytologist · 2017Review
- KETCH1 imports HYL1 to nucleus for miRNA biogenesis inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Coupling Seq-BSA and RNA-Seq Analyses Reveal the Molecular Pathway and Genes Associated with Heading Type in Chinese Cabbage.Frontiers in genetics · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis thaliana HYPONASTIC LEAVES1 (HYL1) is a microRNA (miRNA) biogenesis protein that contains two N-terminal double-stranded RNA binding domains (dsRBDs), a putative nuclear localization site (NLS), and a putative protein-protein interaction domain. The interaction of HYL1 with DICER-LIKE1 is important for the efficient and precise processing of miRNA primary transcripts in plant miRNA biogenesis. To define the roles of the various domains of HYL1 in miRNA processing and the miRNA-directed phenotype, we transferred a series of HYL1 deletion constructs into hyl1 null mutants. The N-terminal region containing dsRBD1 and dsRBD2 completely rescued the mutant phenotype of hyl1, triggering the accumulation of miR166 and miR160 and resulting in reduced mRNA levels of the targeted genes. In vivo biochemical analysis of the HYL1-containing complexes from the transgenic plants revealed that the N-terminal dsRBDs of HYL1 were sufficient for processing miRNA precursors and the generation of mature miRNA. Transient and stable expression analysis demonstrated that the putative NLS domain was indeed the nuclear localization signal, whereas the N-terminal region containing the dsRBDs was not restricted to the nucleus. We suggest that the N-terminal dsRBDs fulfill the function of the whole HYL1 and thus play an essential role in miRNA processing and miRNA-directed silencing of targeted genes.
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