ArticlePlant molecular biology2007
A novel mutated acetolactate synthase gene conferring specific resistance to pyrimidinyl carboxy herbicides in rice.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Enhanced saccharification yields from rice straw by senescence-induced expression of a cytokinin biosynthesis gene in intragenic rice plants.Plant biotechnology (Tokyo, Japan) · 2026Article
- Generating Broad-Spectrum Resistance to ALS-Inhibiting Herbicides in Rice by CRISPR/Cas9-Mediated NHEJ.Rice (New York, N.Y.) · 2025Article
- Advancements of CRISPR-Mediated Base Editing in Crops and Potential Applications inInternational journal of molecular sciences · 2024Review
- Genome-wide association study reveals the genetic basis of rice resistance to three herbicides.Frontiers in plant science · 2024Article
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content.Plant biotechnology journal · 2023Article
- Synthetic evolution of herbicide resistance using a T7 RNAP-based random DNA base editor.Life science alliance · 2022Article
- Transformation of Teosinte (Frontiers in plant science · 2021Article
- Developing dual herbicide tolerant transgenic rice plants for sustainable weed management.Scientific reports · 2018Article
- A novel rice cytochrome P450 gene, CYP72A31, confers tolerance to acetolactate synthase-inhibiting herbicides in rice and Arabidopsis.Plant physiology · 2014Article
- Transformation of apple (Malus × domestica) using mutants of apple acetolactate synthase as a selectable marker and analysis of the T-DNA integration sites.Plant cell reports · 2013Article
- Agrobacterium-mediated co-transformation of rice using two selectable marker genes derived from rice genome components.Plant cell reports · 2012Article
- Enhanced saccharification of rice straw by overexpression of rice exo-glucanase.Rice (New York, N.Y.) · 2012Article
- Selecting genetic transformants of indica and indica-derived rice cultivars using bispyribac sodium and a mutated ALS gene.Plant cell reports · 2010Article
- Selectable tolerance to herbicides by mutated acetolactate synthase genes integrated into the chloroplast genome of tobacco.Plant physiology · 2008Article
- Efficient transformation of wheat by using a mutated rice acetolactate synthase gene as a selectable marker.Plant cell reports · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetolactate synthase (ALS) is the first common enzyme in the biosynthetic pathway of branched-chain amino acids. Mutations of specific amino acids in ALS have been known to confer resistance to ALS-inhibiting herbicides such as sulfonylureas and pyrimidinyl carboxy (PC) herbicides. However, mutations conferring exclusive resistance to PC have not yet been reported to date. We selected PC resistant rice calli, which were derived from anther culture, using one of the PCs, bispyribac-sodium (BS), as a selection agent. Two lines of BS-resistant plants carrying a novel mutation, the 95th Glycine to Alanine (G95A), in ALS were obtained. In vitro ALS activity assay indicated that the recombinant protein of G95A-mutated ALS (ALS-G95A) conferred highly specific resistance to PC herbicides. In order to determine if the ALS-G95A gene could be used as a selection marker for rice transformation, the ALS-G95A gene was connected to ubiquitin promoter and introduced into rice. PC resistant plants containing integrated ALS-G95A gene were obtained after selection with BS as a selection agent. In conclusion, novel G95A mutated ALS gene confers highly specific resistant to PC-herbicides and can be used as a selection marker.
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