ArticlePflugers Archiv : European journal of physiology2007
Calcium indicator loading of neurons using single-cell electroporation.
Article in Pflugers Archiv : European journal of physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Cocaine facilitates CaMolecular psychiatry · 2026Article
- The NaeLife · 2022Article
- High-Efficient and Dosage-Controllable Intracellular Cargo Delivery through Electrochemical Metal-Organic Hybrid Nanogates.Small science · 2021Article
- Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology.Biomolecules · 2021Review
- Review
- Accurate signal-source localization in brain slices by means of high-density microelectrode arrays.Scientific reports · 2019Article
- Optical Quantal Analysis Using CaFrontiers in synaptic neuroscience · 2019Article
- Intracellular CaProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Slow moving neural source in the epileptic hippocampus can mimic progression of human seizures.Scientific reports · 2018Article
- Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes.Nature communications · 2018Article
- Electrochemotherapy of Spinal Metastases Using Transpedicular Approach-A Numerical Feasibility Study.Technology in cancer research & treatment · 2018Article
- Two-Photon Functional Imaging of the Auditory Cortex in Behaving Mice: From Neural Networks to Single Spines.Frontiers in neural circuits · 2018Article
- Glutamate is required for depression but not potentiation of long-term presynaptic function.eLife · 2017Article
- In Vivo Imaging of Microglial Calcium Signaling in Brain Inflammation and Injury.International journal of molecular sciences · 2017Review
- SamuROI, a Python-Based Software Tool for Visualization and Analysis of Dynamic Time Series Imaging at Multiple Spatial Scales.Frontiers in neuroinformatics · 2017Article
- Hippocampal circuit dysfunction in the Tc1 mouse model of Down syndrome.Nature neuroscience · 2015Article
- Reading out a spatiotemporal population code by imaging neighbouring parallel fibre axons in vivo.Nature communications · 2015Article
- Error estimation for reconstruction of neuronal spike firing from fast calcium imaging.Biomedical optics express · 2015Article
- In Vivo Study of Dynamics and Stability of Dendritic Spines on Olfactory Bulb Interneurons in Xenopus laevis Tadpoles.PloS one · 2015Article
- In vivo rapid gene delivery into postmitotic neocortical neurons using iontoporation.Nature protocols · 2015Article
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Authors and funding
2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Studies of subcellular Ca(2+) signaling rely on methods for labeling cells with fluorescent Ca(2+) indicator dyes. In this study, we demonstrate the use of single-cell electroporation for Ca(2+) indicator loading of individual neurons and small neuronal networks in rat neocortex in vitro and in vivo. Brief voltage pulses were delivered through glass pipettes positioned close to target cells. This approach resulted in reliable and rapid (within seconds) loading of somata and subsequent complete labeling of dendritic and axonal arborizations. By using simultaneous whole-cell recordings in brain slices, we directly addressed the effect of electroporation on neurons. Cell viability was high (about 85%) with recovery from the membrane permeabilization occurring within a minute. Electrical properties of recovered cells were indistinguishable before and after electroporation. In addition, Ca(2+) transients with normal appearance could be evoked in dendrites, spines, and axonal boutons of electroporated cells. Using negative-stains of somata, targeted single-cell electroporation was equally applicable in vivo. We conclude that electroporation is a simple approach that permits Ca(2+) indicator loading of multiple cells with low background staining within a short amount of time, which makes it especially well suited for functional imaging of subcellular Ca(2+) dynamics in small neuronal networks.
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