ArticlePlant cell reports2007
Non-antibiotic, efficient selection for alfalfa genetic engineering.
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Cross-Kingdom Communication via Plant-Derived Extracellular Vesicle Nucleic Acids in Genetically EngineeredGenes · 2025Article
- Morphological diversity variation of seed traits among 587 germplasm resources of Medicago Genus and 32 germplasm resources of Trigonella Genus.Scientific reports · 2025Article
- Improved Protocol for EfficientPlants (Basel, Switzerland) · 2024Article
- Exploring Agrobacterium-mediated genetic transformation methods and its applications in Lilium.Plant methods · 2024Review
- Genetic transformation of legumes: an update.Plant cell reports · 2021Review
- Genetic Transformation in Peach (Plants (Basel, Switzerland) · 2020Review
- An Insight into T-DNA Integration Events in Medicago sativa.International journal of molecular sciences · 2017Article
- A mutant Synechococcus gene encoding glutamate 1-semialdehyde aminotransferase confers gabaculine resistance when expressed in tobacco plastids.Plant cell reports · 2015Article
- Assessment of heat shock protein 70 induction by heat in alfalfa varieties and constitutive overexpression in transgenic plants.PloS one · 2015Article
- Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement.Frontiers in plant science · 2014Article
- Assessment of simple marker-free genetic transformation techniques in alfalfa.Plant cell reports · 2011Article
- Modes of action of microbially-produced phytotoxins.Toxins · 2011Review
- Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.Plant cell reports · 2010Review
- Transgenic hybrid aspen overexpressing the Atwbc19 gene encoding an ATP-binding cassette transporter confers resistance to four aminoglycoside antibiotics.Plant cell reports · 2010Article
- An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.).Plant cell reports · 2010Article
- Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.Plant molecular biology · 2010Article
- Evaluation of selection strategies alternative to nptII in genetic transformation of citrus.Plant cell reports · 2008Article
- Bacterial citrate synthase expression and soil aluminum tolerance in transgenic alfalfa.Plant cell reports · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMGs for each plant species is useful for both basic and applied research to combine several genes of interest in the same plant. A selection system based on gabaculine (3-amino-2,3-dihydrobenzoic acid) as the selective substance and the bacterial hemL gene [encoding a mutant for of the enzyme glutamate 1-semialdehyde aminotransferase (GSA-AT)] as the SMG was previously used for genetic transformation of tobacco. The hemL gene is a good candidate for a safe SMG, because GSA-AT is present in all plants and is likely involved in one metabolic step only, so that unintended effects of its overexpression in plants are not probable. In this work, we have compared this new selection system with the conventional, kanamycin-based system for alfalfa Agrobacterium-mediated transformation. The hemL and NptII genes were placed together into a T-DNA under the control of identical promoters and terminators. We show that the gabaculine-based system is more efficient than the conventional, kanamycin-based system. The inheritance of hemL was Mendelian, and no obvious phenotypic effect of its expression was observed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.