ArticleMolecular biology of the cell2007
A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Centrosome migration and apical membrane formation during epithelial polarization in MDCK cysts.eLife · 2026Article
- Spatial regulation of the glycocalyx component podocalyxin is a switch for prometastatic function.Science advances · 2023Article
- Self-organization of apical membrane protein sorting in epithelial cells.The FEBS journal · 2022Article
- Clathrin and clathrin adaptor AP-1 control apical trafficking of megalin in the biosynthetic and recycling routes.Molecular biology of the cell · 2019Article
- Abnormal Golgi pH Homeostasis in Cancer Cells Impairs Apical Targeting of Carcinoembryonic Antigen by Inhibiting Its Glycosyl-Phosphatidylinositol Anchor-Mediated Association with Lipid Rafts.Antioxidants & redox signaling · 2019Article
- A dual role of the extracellular domain ofBiology open · 2018Article
- Loss of mucin-typeThe Journal of biological chemistry · 2017Article
- Newly synthesized and recycling pools of the apical protein gp135 do not occupy the same compartments.Traffic (Copenhagen, Denmark) · 2016Article
- Typhoid toxin provides a window into typhoid fever and the biology of Salmonella Typhi.Proceedings of the National Academy of Sciences of the United States of America · 2016Review
- The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135.The Journal of cell biology · 2015Article
- Trafficking to the apical and basolateral membranes in polarized epithelial cells.Journal of the American Society of Nephrology : JASN · 2014Review
- Exome sequencing and in vitro studies identified podocalyxin as a candidate gene for focal and segmental glomerulosclerosis.Kidney international · 2014Article
- The tails of apical scaffolding proteins EBP50 and E3KARP regulate their localization and dynamics.Molecular biology of the cell · 2013Article
- Membrane trafficking in podocyte health and disease.Pediatric nephrology (Berlin, Germany) · 2013Review
- Article
- Snail destabilizes cell surface Crumbs3a.Traffic (Copenhagen, Denmark) · 2012Article
- PDZ interactions regulate rapid turnover of the scaffolding protein EBP50 in microvilli.The Journal of cell biology · 2012Article
- Mutation conferring apical-targeting motif on AE1 exchanger causes autosomal dominant distal RTA.Journal of the American Society of Nephrology : JASN · 2012Article
- Phosphorylation of EBP50 negatively regulates β-PIX-dependent Rac1 activity in anoikis.Cell death and differentiation · 2012Article
- A hierarchy of signals regulates entry of membrane proteins into the ciliary membrane domain in epithelial cells.The Journal of cell biology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Podocalyxin/Gp135 was recently demonstrated to participate in the formation of a preapical complex to set up initial polarity in MDCK cells, a function presumably depending on the apical targeting of Gp135. We show that correct apical sorting of Gp135 depends on a bipartite signal composed of an extracellular O-glycosylation-rich region and the intracellular PDZ domain-binding motif. The function of this PDZ-binding motif could be substituted with a fusion construct of Gp135 with Ezrin-binding phosphoprotein 50 (EBP50). In accordance with this observation, EBP50 binds to newly synthesized Gp135 at the Golgi apparatus and facilitates oligomerization and sorting of Gp135 into a clustering complex. A defective connection between Gp135 and EBP50 or EBP50 knockdown results in a delayed exit from the detergent-resistant microdomain, failure of oligomerization, and basolateral missorting of Gp135. Furthermore, the basolaterally missorted EBP50-binding defective mutant of Gp135 was rapidly retrieved via a PKC-dependent mechanism. According to these findings, we propose a model by which a highly negative charged transmembrane protein could be packed into an apical sorting platform with the aid of its cytoplasmic partner EBP50.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.