ArticleMolecular biology of the cell2007
Direct binding to Rsp5p regulates ubiquitination-independent vacuolar transport of Sna3p.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Structural insights into the catalysis and regulation of E3 ubiquitin ligases.Nature reviews. Molecular cell biology · 2016Review
- Influence of cellular trafficking pathway on bluetongue virus infection in ovine cells.Viruses · 2015Article
- A family of tetraspans organizes cargo for sorting into multivesicular bodies.Developmental cell · 2015Article
- Article
- ALIX binds a YPX(3)L motif of the GPCR PAR1 and mediates ubiquitin-independent ESCRT-III/MVB sorting.The Journal of cell biology · 2012Article
- Sna3 is an Rsp5 adaptor protein that relies on ubiquitination for its MVB sorting.Traffic (Copenhagen, Denmark) · 2012Article
- The ESCRT complexes.Critical reviews in biochemistry and molecular biology · 2010Review
- Genome-wide approaches to systematically identify substrates of the ubiquitin-proteasome pathway.Trends in biotechnology · 2010Review
- The HECT domain of the ubiquitin ligase Rsp5 contributes to substrate recognition.The Journal of biological chemistry · 2009Article
- Transferrin-directed internalization and cycling of transferrin receptor 2.Traffic (Copenhagen, Denmark) · 2009Article
- Human immunodeficiency virus type 1 Nef protein targets CD4 to the multivesicular body pathway.Journal of virology · 2009Article
- Polyubiquitination by HECT E3s and the determinants of chain type specificity.Molecular and cellular biology · 2009Article
- K63-linked ubiquitin chains as a specific signal for protein sorting into the multivesicular body pathway.The Journal of cell biology · 2009Article
- Membrane protein targeting to the MVB/lysosome.Chemical reviews · 2009Review
- Dual sorting of the Saccharomyces cerevisiae vacuolar protein Sna4p.Eukaryotic cell · 2009Article
- Comparison of substrate specificity of the ubiquitin ligases Nedd4 and Nedd4-2 using proteome arrays.Molecular systems biology · 2009Article
- A C-terminal sequence in the guanine nucleotide exchange factor Sec7 mediates Golgi association and interaction with the Rsp5 ubiquitin ligase.The Journal of biological chemistry · 2008Article
- Hrs and SNX3 functions in sorting and membrane invagination within multivesicular bodies.PLoS biology · 2008Article
- Ubiquitin-dependent virus particle budding without viral protein ubiquitination.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Targeting of Sna3p to the endosomal pathway depends on its interaction with Rsp5p and multivesicular body sorting on its ubiquitylation.Traffic (Copenhagen, Denmark) · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The sorting of integral membrane proteins such as carboxypeptidase S (Cps1p) into the luminal vesicles of multivesicular bodies (MVBs) in Saccharomyces cerevisiae requires ubiquitination of their cytosolic domains by the ubiquitin ligases Rsp5p and/or Tul1p. An exception is Sna3p, which does not require ubiquitination for entry into MVBs. The mechanism underlying this ubiquitination-independent MVB sorting pathway has not yet been characterized. Here, we show that Sna3p sorting into the MVB pathway depends on a direct interaction between a PPAY motif within its C-terminal cytosolic tail and the WW domains of Rsp5p. Disruption of this interaction inhibits vacuolar targeting of Sna3p and causes its accumulation in a compartment that overlaps only partially with MVBs. Surprisingly, Sna3p does require a functional ubiquitin-ligase HECT domain within Rsp5p; however, the dependence of Sna3p on HECT domain activity is distinct from that of Cps1p. Last, we show that Sna3p requires neither Tul1p nor the transmembrane adaptor protein Bsd2p for its MVB sorting. Our data demonstrate that Sna3p follows a novel ubiquitination-independent, but Rsp5p-mediated, sorting pathway to the vacuole.
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