ArticleMolecular biology of the cell2007
Formation of cysts by alveolar type II cells in three-dimensional culture reveals a novel mechanism for epithelial morphogenesis.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
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Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Systematic review of drug effects in humans and models with surfactant-processing disease.European respiratory review : an official journal of the European Respiratory Society · 2018Pooled it
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- Soluble ECM promotes organotypic formation in lung alveolar model.Biomaterials · 2022Article
- GM130 regulates pulmonary surfactant protein secretion in alveolar type II cells.Science China. Life sciences · 2022Article
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- Autophagy inhibition-mediated epithelial-mesenchymal transition augments local myofibroblast differentiation in pulmonary fibrosis.Cell death & disease · 2019Article
- Paracrine signalling during ZEB1-mediated epithelial-mesenchymal transition augments local myofibroblast differentiation in lung fibrosis.Cell death and differentiation · 2019Article
- Biophysical Control of Bile Duct Epithelial Morphogenesis in Natural and Synthetic Scaffolds.Frontiers in bioengineering and biotechnology · 2019Article
- Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease.Nature communications · 2018Article
- Culture of human alveolar epithelial type II cells by sprouting.Respiratory research · 2018Article
- Successful Establishment of Primary Type II Alveolar Epithelium with 3D Organotypic Coculture.American journal of respiratory cell and molecular biology · 2018Article
- Applications of Bioengineered 3D Tissue and Tumor Organoids in Drug Development and Precision Medicine: Current and Future.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2018Review
- Rosiglitazone affects lumen formation in MDCKII cell through regulating apico-basal polarity.American journal of translational research · 2018Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Many organs consist of a hollow cavity surrounded by a monolayer of epithelial cells. Despite their common structure, such organs form by diverse morphogenetic processes. Three-dimensional culture systems have been useful in analyzing the events. Most processes require a combination of cell proliferation and cell death to produce a hollow cavity. Here, we describe a new three-dimensional culture system in which primary human lung alveolar type II cells formed hollow epithelial cysts by a novel process. Individual cells moved, collided, and formed alveolar-like cysts without appreciable proliferation or apoptosis. The alveolar-like cysts consisted of a polarized monolayer of differentiated alveolar type II cells, which secreted surfactant into the central lumen. Blockage of beta1 integrin did not alter cell movement or collision, but it greatly reduced adhesion of cells after collision and subsequent formation of alveolar-like cysts. Treatment of preformed alveolar-like cysts with forskolin increased their diameter, possibly due to stimulation of fluid secretion into the lumen. We conclude that epithelial differentiation and cyst formation can occur without appreciable proliferation or apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.