ArticleBlood2007
Ligand density dramatically affects integrin alpha IIb beta 3-mediated platelet signaling and spreading.
Article in Blood, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 66 citations in OpenAlex.
- Tissue-stiffness-driven platelet activation emerges as a potential target for breast cancer prevention and treatment.Cell communication and signaling : CCS · 2026Article
- Oxygen Concentration Plays a Critical Role in Fibrinogen-Mediated Platelet Activation via Inactivation of αBiomolecules · 2025Article
- Advancing Platelet Research Through Live-Cell Imaging: Challenges, Techniques, and Insights.Sensors (Basel, Switzerland) · 2025Review
- Control of blood capillary networks and holes in blood-brain barrier models by regulating elastic modulus of scaffolds.Materials today. Bio · 2023Article
- Nanofiber Topographies Enhance Platelet-Fibrinogen Scaffold Interactions.Advanced healthcare materials · 2022Article
- Rac Inhibition Causes Impaired GPVI Signalling in Human Platelets through GPVI Shedding and Reduction in PLCγ2 Phosphorylation.International journal of molecular sciences · 2022Article
- Platelets drive fibronectin fibrillogenesis using integrin αIIbβ3.Science advances · 2022Article
- Three-dimensional analysis of blood platelet spreading using digital holographic microscopy: a statistical study of the differential effect of coatings in healthy volunteers and dialyzed patients.Biomedical optics express · 2022Article
- Significant differences in single-platelet biophysics exist across species but attenuate during clot formation.Blood advances · 2021Article
- Blood Clot Phenotyping by Rheometry: Platelets and Fibrinogen Chemistry Affect Stress-Softening and -Stiffening at Large Oscillation Amplitude.Molecules (Basel, Switzerland) · 2020Article
- Differential dynamics of early stages of platelet adhesion and spreading on collagen IV- and fibrinogen-coated surfaces.F1000Research · 2020Article
- The biophysics and mechanics of blood from a materials perspective.Nature reviews. Materials · 2019Article
- Feeling the Force: Measurements of Platelet Contraction and Their Diagnostic Implications.Seminars in thrombosis and hemostasis · 2019Article
- Differential Modulation of Platelet Adhesion and Spreading by Adhesive Ligand Density.Nano letters · 2019Article
- Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals.Scientific reports · 2018Article
- Morphometric analysis of spread platelets identifies integrin αScientific reports · 2018Article
- Platelet integrins exhibit anisotropic mechanosensing and harness piconewton forces to mediate platelet aggregation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Integrin αBlood · 2017Review
- The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) Binder Rasa3 Regulates Phosphoinositide 3-kinase (PI3K)-dependent Integrin αIIbβ3 Outside-in Signaling.The Journal of biological chemistry · 2017Article
- Single-platelet nanomechanics measured by high-throughput cytometry.Nature materials · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The impact of ligand density on integrin-mediated cell adhesion and outside-in signaling is not well understood. Using total internal reflection fluorescent microscopy, conformation-specific antibodies, and Ca(2+) flux measurements, we found that the surface density of fibrinogen affects alpha II b beta 3-mediated platelet signaling, adhesion, and spreading. Adhesion to fibrinogen immobilized at low density leads to rapid increases in cytosolic Ca(2+) and sequential formation of filopodia and lamellipodia. In contrast, adhesion to high-density fibrinogen results in transient or no increases in Ca(2+) and simultaneous formation of filopodia and lamellipodia. alpha II b beta 3 receptors at the basal surface of platelets engage fibrinogen in a ringlike pattern at the cell edges under both conditions. This engagement is, however, more dynamic and easily reversed on high-density fibrinogen. Src and Rac activity and actin polymerization are important for adhesion to low-density fibrinogen, whereas PKC/PI3 kinases contribute to platelet spreading on high-density fibrinogen. We conclude that 2 fundamentally different signaling mechanisms can be initiated by a single integrin receptor interacting with the same ligand when it is immobilized at different densities.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.