ArticleGenes, brain, and behavior2007
Interpretation of knockout experiments: the congenic footprint.
Article in Genes, brain, and behavior, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed.
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- Hibiscetin-Loaded Nanogel Ameliorated the Severity of IMQ-Induced Psoriasis-Like Inflammation in Mice via Down-Regulating Interleukins/TNF-α/NF-κB.Journal of inflammation research · 2026Article
- Genomics of Wolfram Syndrome 1 (WFS1).Biomolecules · 2023Review
- Bridging the translational gap: what can synaptopathies tell us about autism?Frontiers in molecular neuroscience · 2023Review
- Expression Quantitative Trait Loci (eQTLs) Associated with Retrotransposons Demonstrate their Modulatory Effect on the Transcriptome.International journal of molecular sciences · 2021Article
- At the dawn of the transcriptomic medicine.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- A modifier in the 129S2/SvPasCrl genome is responsible for the viability of Notch1[12f/12f] mice.BMC developmental biology · 2019Article
- Streamlined computational pipeline for genetic background characterization of genetically engineered mice based on next generation sequencing data.BMC genomics · 2019Article
- A 30-year journey from volume-regulated anion currents to molecular structure of the LRRC8 channel.The Journal of general physiology · 2019Review
- Signatures of Altered Gene Expression in Dorsal Root Ganglia of a Fabry Disease Mouse Model.Frontiers in molecular neuroscience · 2017Article
- Gene Targeting Using Homologous Recombination in Embryonic Stem Cells: The Future for Behavior Genetics?Frontiers in genetics · 2016Review
- Effect of light on global gene expression in the neuroglobin-deficient mouse retina.Biomedical reports · 2014Article
- Altered gene expression patterns in muscle ring finger 1 null mice during denervation- and dexamethasone-induced muscle atrophy.Physiological genomics · 2013Article
- Spontaneous metastasis in congenic mice with transgenic breast cancer is unaffected by plasminogen gene ablation.Clinical & experimental metastasis · 2013Article
- Increased placental expression and maternal serum levels of apoptosis-inducing TRAIL in recurrent miscarriage.Placenta · 2013Article
- Limbic system associated membrane protein as a potential target for neuropsychiatric disorders.Frontiers in pharmacology · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In gene targeting experiments, the importance of genetic background is now widely appreciated, and knockout alleles are routinely backcrossed onto a standard inbred background. This produces a congenic strain with a substantial segment of embryonic stem (ES)-cell-derived chromosome still flanking the knockout allele, a phenomenon often neglected in knockout studies. In cholecystokynin 2 (Cckbr) knockout mice backcrossed with C57BL/6, we have found a clear 'congenic footprint' of expression differences in at least 10 genes across 40 Mb sequence flanking the Cckbr locus, each of which is potentially responsible for aspects of the 'knockout' phenotype. The expression differences are overwhelmingly in the knockout-low direction, which may point to a general phenomenon of background dependence. This finding emphasizes the need for caution in using gene knockouts to attribute phenotypic effects to genes. This is especially the case when the gene is of unknown function or the phenotype is unexpected, and is a particular concern for large-scale knockout and phenotypic screening programmes. However, the impact of genetic background should not be simply viewed as a potential confound, but as a unique opportunity to study the broader responses of a system to a specific (genetic) perturbation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.