ArticleToxicological sciences : an official journal of the Society of Toxicology2007
Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 74 citations in OpenAlex.
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- Differential response to hepatic differentiation stimuli of amniotic epithelial cells isolated from four regions of the amniotic membrane.Journal of cellular and molecular medicine · 2020Article
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- Bioinformatic analysis of microRNA networks following the activation of the constitutive androstane receptor (CAR) in mouse liver.Biochimica et biophysica acta · 2016Article
- Cell sources for in vitro human liver cell culture models.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Hepatic Aryl Hydrocarbon Receptor Attenuates Fibroblast Growth Factor 21 Expression.The Journal of biological chemistry · 2016Article
- A three-dimensional collagen-sponge-based culture system coated with simplified recombinant fibronectin improves the function of a hepatocyte cell line.In vitro cellular & developmental biology. Animal · 2016Article
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- Biomarker: the universe of chemically induced gene expression alterations in human hepatocyte.EXCLI journal · 2014Article
- Type XVIII collagen is essential for survival during acute liver injury in mice.Disease models & mechanisms · 2013Article
- Induction of albumin expression in HepG2 cells using immobilized simplified recombinant fibronectin protein.In vitro cellular & developmental biology. Animal · 2013Article
- Expression of a novel mRNA transcript for human microsomal epoxide hydrolase (EPHX1) is regulated by short open reading frames within its 5'-untranslated region.RNA (New York, N.Y.) · 2013Article
- In vitro toxicity assessment of amphiphillic polymer-coated CdSe/ZnS quantum dots in two human liver cell models.ACS nano · 2012Article
- Multi-species analyses of direct activators of the constitutive androstane receptor.Toxicological sciences : an official journal of the Society of Toxicology · 2011Article
- Differentiation of human embryonic stem cells along a hepatic lineage.Chemico-biological interactions · 2011Article
- Differentiation of bone marrow-derived mesenchymal stem cells into hepatocyte-like cells on nanofibers and their transplantation into a carbon tetrachloride-induced liver fibrosis model.Stem cell reviews and reports · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Previously, we demonstrated that primary cultures of rat hepatocytes evidence higher levels of differentiated function when cultured in the presence of a dilute overlay of extracellular matrix (Matrigel). In this investigation, we used DNA microarrays, quantitative RT-PCR, immunoblotting, and cell morphology analyses to evaluate the biological responses imparted by Matrigel overlays on primary cultures of human hepatocytes from five independent donors. Although interindividual variability in responses was evident, our results demonstrated that Matrigel additions typically improved hepatocyte morphology and differentiation character. Results from RNA-profiling experiments indicated that Matrigel additions enhanced hepatocyte RNA expression levels associated with a battery of differentiated features, to levels comparable to those seen in vivo, for genes such as the cytochrome P450s, solute carrier family members, sulfotransferases, certain nuclear transcription factors, and other liver-specific markers, such as albumin, transferrin, and response to the inducer, phenobarbital. In contrast, Matrigel additions were generally associated with reduced RNA expression levels for several cytokeratins, integrins, and a number of stress-related pathways. Decreases in integrin protein expression were similarly detected, although enhanced levels of the gap junction-associated protein, connexin 32, were detected in Matrigel-treated cultures. These data support the concept that ECM functions mechanistically to augment the differentiation character of primary human hepatocytes in culture by mediating a reduction in cellular stress response signaling and by enhancing gap junctional cell-cell communication.
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