ArticleBMC microbiology2007
A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Recombinant Haemagglutinin Derived From the Ciliated ProtozoanFrontiers in immunology · 2019Article
- System for the heterologous expression of NS1 protein of H9N2 avian influenza virus in the ciliate Tetrahymena thermophila.The Journal of veterinary medical science · 2018Article
- Article
- The two human centrin homologues have similar but distinct functions at Tetrahymena basal bodies.Molecular biology of the cell · 2012Article
- Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.Methods in cell biology · 2012Article
- The two domains of centrin have distinct basal body functions in Tetrahymena.Molecular biology of the cell · 2011Article
- Production of recombinant proteins from protozoan parasites.Trends in parasitology · 2010Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses we developed a Cre-dependent recombinase system.
resultsWe created novel donor and acceptor vectors that facilitate the transfer of expression cassettes from the donor into novel acceptor plasmid. Expression vectors were used that encode the 19 kDa C-terminus of the MSP1 protein of Plasmodium falciparum and a blasticidin S (bsdR) resistance gene, respectively. The functional expression of these genes was demonstrated by western blot analysis with MSP1 specific antibodies and by a blasticidin growing assay.
conclusionThe Cre dependent recombinase system in combination with the modular structure of the donor vectors ease cloning and expression of foreign genes in the ciliate system, providing a powerful tool for protistology research in future.
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