ArticleThe European journal of neuroscience2007
Hydrogen peroxide increases GABAergic mIPSC through presynaptic release of calcium from IP3 receptor-sensitive stores in spinal cord substantia gelatinosa neurons.
Article in The European journal of neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 50 citations in OpenAlex.
- The development but not the maintenance of phrenic and sympathetic long-term facilitation after acute intermittent hypoxia requires nucleus tractus solitarii HExperimental neurology · 2025Article
- Phosphorylation of GABAScientific reports · 2025Article
- Hydrogen peroxide suppresses excitability of gonadotropin-releasing hormone neurons in adult mouse.Frontiers in endocrinology · 2022Article
- Mitochondrial hydrogen peroxide positively regulates neuropeptide secretion during diet-induced activation of the oxidative stress response.Nature communications · 2021Article
- Anti-hypertensive effect of hydrogen peroxide acting centrally.Hypertension research : official journal of the Japanese Society of Hypertension · 2020Article
- Multiple sources of internal calcium stores mediate ethanol-induced presynaptic inhibitory GABA release in the central nucleus of the amygdala in mice.Psychopharmacology · 2020Article
- Hydrogen peroxide inhibits neurons in the paraventricular nucleus of the hypothalamus via potassium channel activation.American journal of physiology. Regulatory, integrative and comparative physiology · 2019Article
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- Promoter-Specific Effects of DREADD Modulation on Hippocampal Synaptic Plasticity and Memory Formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Hydrogen peroxide modulates synaptic transmission in ventral horn neurons of the rat spinal cord.The Journal of physiology · 2016Article
- Reactive Oxygen Species Donors Increase the Responsiveness of Dorsal Horn Neurons and Induce Mechanical Hyperalgesia in Rats.Neural plasticity · 2015Article
- Hydrogen peroxide increases GABAA receptor-mediated tonic current in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- An intracellular redox sensor for reactive oxygen species at the M3-M4 linker of GABAA ρ1 receptors.British journal of pharmacology · 2014Article
- H₂O₂ induces delayed hyperexcitability in nucleus tractus solitarii neurons.Neuroscience · 2014Article
- Effect of antioxidant treatment on spinal GABA neurons in a neuropathic pain model in the mouse.Pain · 2013Article
- Advanced glycation end products acutely impair ca(2+) signaling in bovine aortic endothelial cells.Frontiers in physiology · 2013Article
- Modulation of Presynaptic GABA Release by Oxidative Stress in Mechanically-isolated Rat Cerebral Cortical Neurons.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2010Article
- SR/ER-mitochondrial local communication: calcium and ROS.Biochimica et biophysica acta · 2009Review
- H(2)O(2)-mediated modulation of cytosolic signaling and organelle function in rat hippocampus.Pflugers Archiv : European journal of physiology · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
GABAergic interneurons of the spinal cord substantia gelatinosa regulate the transmission of nociceptive information. Hydrogen peroxide (H2O2) is likely a diffusible messenger contributing to the development of long-lasting pathological pain states after nerve injury. In this study, we examined the presynaptic effects of H2O2 on the inhibitory interneurons of mouse substantia gelatinosa (SG) using whole-cell patch-clamp recordings from spinal cord slices. H2O2 increased the frequency of GABAergic miniature inhibitory postsynaptic current (mIPSC) in a concentration-dependent (10-1000 microM) manner. The profound increase in mIPSC frequency was diminished by thapsigargin or cyclopiazonic acid suggesting that the intracellular stored pool was the source of presynaptic calcium. Further examination revealed the 2-aminoethoxydiphenil borate blockable inositol-(1,4,5) trisphosphate receptor (IP3R) regulated pool of stored calcium as the likely source. The phospholipase C (PLC) blocker, 1-(6-[([17beta]-3-methoxyestra-1,3,5[10]-trien-17-yl)-amino]hexyl)-1H-pyrrole-2,5-dione (U73122), did not block the frequency increase, which suggested that the site of action of H2O2 lies downstream in the IP3 signalling pathway, and nifedipine-sensitivity of the frequency increase indicated a possible role of calcium-induced calcium-release. However, a direct examination of L-type voltage-gated calcium channels (VGCC) demonstrated that H2O2 did not increase the calcium influx through these channels. The H2O2 effect on mIPSC frequency was markedly reduced in the opisthotonus (Opt) mutant mice with a known deletion in the IP3R1 gene. We demonstrated that H2O2 increased presynaptic activity in the GABAergic interneurons by the release of calcium from the IP3R-regulated intracellular pool. The presynaptic IP3R could emerge as a novel target for preventing H2O2-induced synaptic plasticity in substantia gelatinosa leading to pathological pain states.
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