ArticleExperimental biology and medicine (Maywood, N.J.)2007
Gene transfer of interleukin 10 to the murine cornea using electroporation.
Article in Experimental biology and medicine (Maywood, N.J.), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Viral microRNA inhibition enhances antiviral immunity by modulating corneal inflammatory and resolution pathways in HSV-1 induced keratitis.Experimental eye research · 2026Article
- Role of NADPH Oxidase 4 in Corneal Endothelial Cells Is Mediated by Endoplasmic Reticulum Stress and Autophagy.Antioxidants (Basel, Switzerland) · 2023Article
- Review
- Gene Therapy for Acute Respiratory Distress Syndrome.Frontiers in physiology · 2021Review
- Non-viral Gene Delivery Methods for Bone and Joints.Frontiers in bioengineering and biotechnology · 2020Review
- Physical energy for drug delivery; poration, concentration and activation.Advanced drug delivery reviews · 2014Review
- Corneal gene therapy: basic science and translational perspective.The ocular surface · 2013Review
- Systematic assessment of microneedle injection into the mouse cornea.European journal of medical research · 2012Article
- Targeting herpetic keratitis by gene therapy.Journal of ophthalmology · 2012Article
- Gene therapy in the cornea: 2005--present.Progress in retinal and eye research · 2012Review
- Gene transfer: how can the biological barriers be overcome?The Journal of membrane biology · 2010Article
- Gene delivery to cornea.Brain research bulletin · 2010Review
- Electrically assisted delivery of macromolecules into the corneal epithelium.Experimental eye research · 2009Article
- Efficient lentiviral gene transfer into corneal stroma cells using a femtosecond laser.Gene therapy · 2009Article
- Intracellular trafficking of plasmids for gene therapy: mechanisms of cytoplasmic movement and nuclear import.Current gene therapy · 2006Review
- Strategies for local gene therapy of corneal allograft rejection.Middle East African journal of ophthalmologyReview
- Gene therapy in keratoconus.Oman journal of ophthalmologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Gene therapy is a promising approach to deliver anti-inflammatory genes to the eye to treat a number of corneal diseases. We have used electroporation to deliver plasmids expressing interleukin 10 (IL-10) to the corneas of mice and evaluated the duration of expression following gene transfer. Unlike expression of reporter genes driven from the cytomegalovirus immediate early promoter (CMV(iep)), which remained high for 3 days, CMV(iep)-driven IL-10 expression peaked at Day 1 and decreased 2-fold each day thereafter. In an attempt to increase the duration of expression, the long-acting ubiquitin C (UbC) promoter was used but, surprisingly, a similar half-life of gene expression was observed. This reduced duration was not due to promoter inhibition by expressed IL-10 or clearance of plasmids from the cornea. However, when DNA nuclear targeting sequences (DTSs) that promote DNA nuclear import were removed from the plasmids, contrary to findings in nondividing cells and tissues in which these sequences are needed for gene transfer, robust expression was observed, and the duration increased significantly. Although corneal cell turnover was detected, suggesting mitosis-dependent plasmid nuclear localization independent of a DTS, the patterns of expressing and dividing cells appeared different. These results suggest that DNA nuclear targeting sequences may act differently in the cornea than in other tissues.
Indexed as
Identifiers
17327469PMC4403727W1855220807What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.