ArticleAntimicrobial agents and chemotherapy2007
Selective phosphorylation of antiviral drugs by vaccinia virus thymidine kinase.
Article in Antimicrobial agents and chemotherapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Single-day famciclovir for the treatment of genital herpes: follow-up results of time to next recurrence and assessment of antiviral resistance.Current medical research and opinion · 2009Trial
- Monkeypox: Origin, Transmission, Clinical Manifestations, Prevention, and Therapeutic Options.Interdisciplinary perspectives on infectious diseases · 2025Review
- Oncolytic virotherapy for hepatocellular carcinoma: A potent immunotherapeutic landscape.World journal of gastrointestinal oncology · 2024Article
- Engineering and Characterization of Oncolytic Vaccinia Virus Expressing Truncated Herpes Simplex Virus Thymidine Kinase.Cancers · 2020Article
- Orthopoxvirus targets for the development of new antiviral agents.Antiviral research · 2012Review
- Identification of protein-protein interaction inhibitors targeting vaccinia virus processivity factor for development of antiviral agents.Antimicrobial agents and chemotherapy · 2011Article
- Radiolabeled antiviral drugs and antibodies as virus-specific imaging probes.Antiviral research · 2010Review
- Article
- Vaccinia virus-encoded ribonucleotide reductase subunits are differentially required for replication and pathogenesis.PLoS pathogens · 2010Article
- Inhibition of herpesvirus replication by 5-substituted 4'-thiopyrimidine nucleosides.Antimicrobial agents and chemotherapy · 2009Article
- Activities of certain 5-substituted 4'-thiopyrimidine nucleosides against orthopoxvirus infections.Antimicrobial agents and chemotherapy · 2009Article
- Crystal structure of poxvirus thymidylate kinase: an unexpected dimerization has implications for antiviral therapy.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Therapeutic and prophylactic drugs to treat orthopoxvirus infections.Future virology · 2008Article
- Isolation and characterization of cidofovir resistant vaccinia viruses.Virology journal · 2008Article
- Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug (N)-methanocarbathymidine.Virology journal · 2008Article
- Identification of multiple independent horizontal gene transfers into poxviruses using a comparative genomics approach.BMC evolutionary biology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
The antiviral activity of a new series of thymidine analogs was determined against vaccinia virus (VV), cowpox virus (CV), herpes simplex virus, and varicella-zoster virus. Several compounds were identified that had good activity against each of the viruses, including a set of novel 5-substituted deoxyuridine analogs. To investigate the possibility that these drugs might be phosphorylated preferentially by the viral thymidine kinase (TK) homologs, the antiviral activities of these compounds were also assessed using TK-deficient strains of some of these viruses. Some of these compounds were shown to be much less effective in the absence of a functional TK gene in CV, which was unexpected given the high degree of amino acid identity between this enzyme and its cellular homolog. This unanticipated result suggested that the CV TK was important in the mechanism of action of these compounds and also that it might phosphorylate a wider variety of substrates than other type II enzymes. To confirm these data, we expressed the VV TK and human TK1 in bacteria and isolated the purified enzymes. Enzymatic assays demonstrated that the viral TK could efficiently phosphorylate many of these compounds, whereas most of the compounds were very poor substrates for the cellular kinase, TK1. Thus, the specific phosphorylation of these compounds by the viral kinase may be sufficient to explain the TK dependence. This unexpected result suggests that selective phosphorylation by the viral kinase may be a promising new approach in the discovery of highly selective inhibitors of orthopoxvirus replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.