ArticleInfection and immunity2007
Characterization of SodC, a periplasmic superoxide dismutase from Burkholderia cenocepacia.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- Gasdermin D restricts Burkholderia cenocepacia infection in vitro and in vivo.Scientific reports · 2021Article
- Low iron-induced small RNA BrrF regulates central metabolism and oxidative stress responses in Burkholderia cenocepacia.PloS one · 2020Article
- Article
- Using dendritic cells to evaluate how Burkholderia cenocepacia clonal isolates from a chronically infected cystic fibrosis patient subvert immune functions.Medical microbiology and immunology · 2017Article
- Comparative genome-wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis.MicrobiologyOpen · 2016Article
- Antioxidant enzymes activities of Burkholderia spp. strains-oxidative responses to Ni toxicity.Environmental science and pollution research international · 2015Article
- Comparative transcriptomic analysis of the Burkholderia cepacia tyrosine kinase bceF mutant reveals a role in tolerance to stress, biofilm formation, and virulence.Applied and environmental microbiology · 2013Article
- Expression and characterization of an iron-containing superoxide dismutase from Burkholderia pseudomallei.Journal of microbiology (Seoul, Korea) · 2012Article
- Influence of neutrophil defects on Burkholderia cepacia complex pathogenesis.Frontiers in cellular and infection microbiology · 2011Review
- A decade of Burkholderia cenocepacia virulence determinant research.Infection and immunity · 2010Review
- Oxidative stress of Burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping.Journal of clinical microbiology · 2010Article
- The TolC protein of Legionella pneumophila plays a major role in multi-drug resistance and the early steps of host invasion.PloS one · 2009Article
- Steps toward broad-spectrum therapeutics: discovering virulence-associated genes present in diverse human pathogens.BMC genomics · 2009Article
- Regulatory and structural properties differentiating the chromosomal and the bacteriophage-associated Escherichia coli O157:H7 Cu, Zn superoxide dismutases.BMC microbiology · 2008Article
- Burkholderia cenocepacia requires the RpoN sigma factor for biofilm formation and intracellular trafficking within macrophages.Infection and immunity · 2008Article
- Burkholderia cenocepacia C5424 produces a pigment with antioxidant properties using a homogentisate intermediate.Journal of bacteriology · 2007Article
- Burkholderia cenocepacia infection: disruption of phagocyte immune functions through Rho GTPase inactivation.Cell adhesion & migrationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia cenocepacia is a gram-negative, non-spore-forming bacillus and a member of the Burkholderia cepacia complex. B. cenocepacia can survive intracellularly in phagocytic cells and can produce at least one superoxide dismutase (SOD). The inability of O2- to cross the cytoplasmic membrane, coupled with the periplasmic location of Cu,ZnSODs, suggests that periplasmic SODs protect bacteria from superoxide that has an exogenous origin (for example, when cells are faced with reactive oxygen intermediates generated by host cells in response to infection). In this study, we identified the sodC gene encoding a Cu,ZnSOD in B. cenocepacia and demonstrated that a sodC null mutant was not sensitive to a H2O2, 3-morpholinosydnonimine, or paraquat challenge but was killed by exogenous superoxide generated by the xanthine/xanthine oxidase method. The sodC mutant also exhibited a growth defect in liquid medium compared to the parental strain, which could be complemented in trans. The mutant was killed more rapidly than the parental strain was killed in murine macrophage-like cell line RAW 264.7, but killing was eliminated when macrophages were treated with an NADPH oxidase inhibitor. We also confirmed that SodC is periplasmic and identified the metal cofactor. B. cenocepacia SodC was resistant to inhibition by H2O2 and was unusually resistant to KCN for a Cu,ZnSOD. Together, these observations establish that B. cenocepacia produces a periplasmic Cu,ZnSOD that protects this bacterium from exogenously generated O2- and contributes to intracellular survival of this bacterium in macrophages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.