ArticleBiophysical journal2007
(Un)confined diffusion of CD59 in the plasma membrane determined by high-resolution single molecule microscopy.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 132 citations in OpenAlex.
- Flow transports extracellular lipid-anchored proteins across the surface of living COS-7 cells.Biophysical journal · 2026Article
- Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift.Nature communications · 2025Article
- High-Speed Interferometric Scattering Tracking Microscopy of Compartmentalized Lipid Diffusion in Living Cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Single-photon microscopy to study biomolecular condensates.Nature communications · 2023Article
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
- Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology.The Journal of cell biology · 2023Article
- Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Influence of the extracellular domain size on the dynamic behavior of membrane proteins.Biophysical journal · 2022Article
- Heterogeneous nanoscopic lipid diffusion in the live cell membrane and its dependency on cholesterol.Biophysical journal · 2022Article
- Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins.ACS nano · 2021Article
- DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.Immunity · 2021Article
- TRAIT2D: a Software for Quantitative Analysis of Single Particle Diffusion Data.F1000Research · 2021Article
- Inefficient CAR-proximal signaling blunts antigen sensitivity.Nature immunology · 2020Article
- Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells.Molecular biology of the cell · 2020Article
- Review
- Review
- Article
- Membrane Diffusion Occurs by Continuous-Time Random Walk Sustained by Vesicular Trafficking.Biophysical journal · 2018Article
- High-Speed Single-Molecule Tracking of CXCL13 in the B-Follicle.Frontiers in immunology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
There has been emerging interest whether plasma membrane constituents are moving according to free Brownian motion or hop diffusion. In the latter model, lipids, lipid-anchored proteins, and transmembrane proteins would be transiently confined to periodic corrals in the cell membrane, which are structured by the underlying membrane skeleton. Because this model is based exclusively on results provided by one experimental strategy--high-resolution single particle tracking--we attempted in this study to confirm or amend it using a complementary technique. We developed a novel strategy that employs single molecule fluorescence microscopy to detect confinements to free diffusion of CD59--a GPI-anchored protein--in the plasma membrane of living T24 (ECV) cells. With this method, minimum invasive labeling via fluorescent Fab fragments was sufficient to measure the lateral motion of individual protein molecules on a millisecond timescale, yielding a positional accuracy down to 22 nm. Although no hop diffusion was directly observable, based on a full analytical description our results provide upper boundaries for confinement size and strength.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.