ArticleBiophysical journal2007
Fluorescence microscopy evidence for quasi-permanent attachment of antifreeze proteins to ice surfaces.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 119 citations in OpenAlex.
- On the engulfment of antifreeze proteins by ice.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Extended Temperature Range of the Ice-Binding Protein Activity.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Divergent Mechanisms of Ice Growth Inhibition by Antifreeze Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Nanoscopy of single antifreeze proteins reveals that reversible ice binding is sufficient for ice recrystallization inhibition but not thermal hysteresis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Adsorption of ice-binding proteins onto whole ice crystal surfaces does not necessarily confer a high thermal hysteresis activity.Scientific reports · 2022Article
- Origin of an antifreeze protein gene in response to Cenozoic climate change.Scientific reports · 2022Article
- Intracellular and Extracellular Antifreeze Protein Significantly Improves Mammalian Cell Cryopreservation.Biomolecules · 2022Article
- Ice Binding Proteins: Diverse Biological Roles and Applications in Different Types of Industry.Biomolecules · 2020Review
- Peptidic Antifreeze Materials: Prospects and Challenges.International journal of molecular sciences · 2019Review
- From ice-binding proteins to bio-inspired antifreeze materials.Soft matter · 2017Review
- Article
- Microfluidic Cold-Finger Device for the Investigation of Ice-Binding Proteins.Biophysical journal · 2016Article
- Putting life on ice: bacteria that bind to frozen water.Journal of the Royal Society, Interface · 2016Article
- Article
- Ice-binding proteins that accumulate on different ice crystal planes produce distinct thermal hysteresis dynamics.Journal of the Royal Society, Interface · 2014Article
- Molecular recognition of methyl α-D-mannopyranoside by antifreeze (glyco)proteins.Journal of the American Chemical Society · 2014Article
- Determining the ice-binding planes of antifreeze proteins by fluorescence-based ice plane affinity.Journal of visualized experiments : JoVE · 2014Article
- Bacterial ice crystal controlling proteins.Scientifica · 2014Review
- LabVIEW-operated novel nanoliter osmometer for ice binding protein investigations.Journal of visualized experiments : JoVE · 2013Article
- Microfluidic experiments reveal that antifreeze proteins bound to ice crystals suffice to prevent their growth.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many organisms are protected from freezing by the presence of extracellular antifreeze proteins (AFPs), which bind to ice, modify its morphology, and prevent its further growth. These proteins have a wide range of applications including cryopreservation, frost protection, and as models in biomineralization research. However, understanding their mechanism of action remains an outstanding challenge. While the prevailing adsorption-inhibition hypothesis argues that AFPs must bind irreversibly to ice to arrest its growth, other theories suggest that there is exchange between the bound surface proteins and the free proteins in solution. By conjugating green fluorescence protein (GFP) to a fish AFP (Type III), we observed the binding of the AFP to ice. This was accomplished by monitoring the presence of GFP-AFP on the surface of ice crystals several microns in diameter using fluorescence microscopy. The lack of recovery of fluorescence after photobleaching of the GFP component of the surface-bound GFP-AFP shows that there is no equilibrium surface-solution exchange of GFP-AFP and thus supports the adsorption-inhibition mechanism for this type of AFP. Moreover, our study establishes the utility of fluorescently labeled AFPs as a research tool for investigating the mechanisms underlying the activity of this diverse group of proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.