ArticleBiophysical journal2007
Modeling of spatial metabolite distributions in the cardiac sarcomere.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Co-localization and confinement of ecto-nucleotidases modulate extracellular adenosine nucleotide distributions.PLoS computational biology · 2020Article
- Enzyme Fusion Removes Competition for Geranylgeranyl Diphosphate in Carotenogenesis.Plant physiology · 2019Article
- Intracellular Energy-Transfer Networks and High-Resolution Respirometry: A Convenient Approach for Studying Their Function.International journal of molecular sciences · 2018Review
- Molecular and subcellular-scale modeling of nucleotide diffusion in the cardiac myofilament lattice.Biophysical journal · 2013Article
- Mechanisms of altered Ca²⁺ handling in heart failure.Circulation research · 2013Review
- ATP binding and cross-bridge detachment steps during full Ca²⁺ activation: comparison of myofibril and muscle fibre mechanics by sinusoidal analysis.The Journal of physiology · 2012Article
- Compartmentation of membrane processes and nucleotide dynamics in diffusion-restricted cardiac cell microenvironment.Journal of molecular and cellular cardiology · 2012Review
- A three-dimensional simulation model of cardiomyocyte integrating excitation-contraction coupling and metabolism.Biophysical journal · 2011Article
- Compartmentation of glycogen metabolism revealed from 13C isotopologue distributions.BMC systems biology · 2011Article
- Molecular system bioenergics of the heart: experimental studies of metabolic compartmentation and energy fluxes versus computer modeling.International journal of molecular sciences · 2011Review
- Application of the principles of systems biology and Wiener's cybernetics for analysis of regulation of energy fluxes in muscle cells in vivo.International journal of molecular sciences · 2010Review
- Strong inference for systems biology.PLoS computational biology · 2009Article
- Anisotropic diffusion of fluorescently labeled ATP in rat cardiomyocytes determined by raster image correlation spectroscopy.American journal of physiology. Cell physiology · 2008Article
- Metabolic compartmentation - a system level property of muscle cells: real problems of diffusion in living cells.International journal of molecular sciences · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although a high ATP diffusion rate implies homogeneous distribution of the principal energetic currency in the cytosol, local diffusion barriers represented by macromolecular structures can render ATP concentrations to be inhomogeneous. A method is presented here that provides apparent diffusion coefficient values in local intracellular regions and allows the estimation of spatial metabolite distribution. The apparent local diffusion coefficient for ATP in cardiac myofibrils was determined from the analysis of diffusion-dependent rightward shift of the substrate dependence for actomyosin ATPase activity using the reaction-diffusion model, which accounted for the properties of phosphotransfer reactions. This functional analysis, which took into account the local diffusional ATP delivery to the active sites, provided an apparent value that was three orders of magnitude lower than that defined by direct methods for the cytosol. The low value of the diffusion coefficient was shown to define unusual properties of the intracellular space in working heart, where small reductions in ATP levels in the surrounding cytosol result in a large drop in [ATP] inside myofibrils. This drop is critical for vital cellular functions, and the analysis presented here defines its physical basis. The diffusion barriers thus defined explain the coexistence of pathological energy deficit with almost normal average ATP levels.
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