ArticleJournal of veterinary science2007
Organotypic slice culture of the hypothalamic paraventricular nucleus of rat.
Article in Journal of veterinary science, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Structure and hormonal output of the adrenal gland after experimental estrogenization of male rats.Histology and histopathology · 2025Article
- Photoswitchable Probes of Oxytocin and Vasopressin.Journal of medicinal chemistry · 2023Article
- Organotypic Spinal Cord Culture: a Proper Platform for the Functional Screening.Molecular neurobiology · 2016Review
- Effects of blast overpressure on neurons and glial cells in rat organotypic hippocampal slice cultures.Frontiers in neurology · 2015Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.
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