ArticleEukaryotic cell2007
Basic helix-loop-helix transcription factor heterocomplex of Yas1p and Yas2p regulates cytochrome P450 expression in response to alkanes in the yeast Yarrowia lipolytica.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 65 citations in OpenAlex.
- Utilization, metabolic regulation and applications of hydrophobic substrates inSynthetic and systems biotechnology · 2026Review
- Phenotyping transcription factors-related genotypes in Y. lipolytica across a range of industrially relevant process parameters and chemical stress conditions.Microbial cell factories · 2026Article
- Transcriptional regulation: efficient genetic engineering tools for non-conventional yeasts.FEMS yeast research · 2026Review
- Global transcription machinery engineering in Yarrowia lipolytica.FEMS yeast research · 2025Review
- Transcriptional activation and coactivator binding by yeast Ino2 and human proto-oncoprotein c-Myc.Current genetics · 2025Article
- An Interplay between Transcription Factors and Recombinant Protein Synthesis inInternational journal of molecular sciences · 2024Article
- Mar1, a high mobility group box protein, regulatesApplied and environmental microbiology · 2024Article
- Article
- 'Mother(Nature) knows best' - hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database.Microbial cell factories · 2024Article
- Phosphatidylserine synthase plays a critical role in the utilization of n-alkanes in the yeast Yarrowia lipolytica.FEMS yeast research · 2024Article
- Transcription factors enhancing synthesis of recombinant proteins and resistance to stress in Yarrowia lipolytica.Applied microbiology and biotechnology · 2023Article
- Utilization of n-alkane and roles of lipid transfer proteins in Yarrowia lipolytica.World journal of microbiology & biotechnology · 2023Review
- Insights into the Genomic and Phenotypic Landscape of the Oleaginous YeastJournal of fungi (Basel, Switzerland) · 2023Article
- A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Analysis ofMetabolic engineering communications · 2020Article
- Article
- The Basic-Region Helix-Loop-Helix Transcription Factor DevR Significantly Affects Polysaccharide Metabolism in Aspergillus oryzae.Applied and environmental microbiology · 2019Article
- Increasing medium chain fatty acids production in Yarrowia lipolytica by metabolic engineering.Microbial cell factories · 2018Article
- Development of a promising microbial platform for the production of dicarboxylic acids from biorenewable resources.Biotechnology for biofuels · 2018Article
- Fatty aldehyde dehydrogenase multigene family involved in the assimilation of n-alkanes in Yarrowia lipolytica.The Journal of biological chemistry · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the ALK1 gene, which encodes cytochrome P450, catalyzing the first step of alkane oxidation in the alkane-assimilating yeast Yarrowia lipolytica, is highly regulated and can be induced by alkanes. Previously, we identified a cis-acting element (alkane-responsive element 1 [ARE1]) in the ALK1 promoter. We showed that a basic helix-loop-helix (bHLH) protein, Yas1p, binds to ARE1 in vivo and mediates alkane-dependent transcription induction. Yas1p, however, does not bind to ARE1 by itself in vitro, suggesting that Yas1p requires another bHLH protein partner for its DNA binding, as many bHLH transcription factors function by forming heterodimers. To identify such a binding partner of Yas1p, here we screened open reading frames encoding proteins with the bHLH motif from the Y. lipolytica genome database and identified the YAS2 gene. The deletion of the YAS2 gene abolished the alkane-responsive induction of ALK1 transcription and the growth of the yeast on alkanes. We revealed that Yas2p has transactivation activity. Furthermore, Yas1p and Yas2p formed a protein complex that was required for the binding of these proteins to ARE1. These findings allow us to postulate a model in which bHLH transcription factors Yas1p and Yas2p form a heterocomplex and mediate the transcription induction in response to alkanes.
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