ArticleApplied and environmental microbiology2007
Comparative high-density microarray analysis of gene expression during growth of Lactobacillus helveticus in milk versus rich culture medium.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 94 citations in OpenAlex.
- The Enzyme Gene Expression of Protein Utilization and Metabolism byMicroorganisms · 2022Article
- Visualization of Gene Reciprocity among Lactic Acid Bacteria in Yogurt by RNase H-Assisted Rolling Circle Amplification-FluorescenceMicroorganisms · 2021Article
- Integrated Transcriptome and Proteome Analyses Reveal Protein Metabolism inFrontiers in microbiology · 2021Article
- Proteomic Analysis ofFrontiers in microbiology · 2019Article
- Distribution of Cell Envelope Proteinases Genes among Polish Strains of Lactobacillus helveticus.Polish journal of microbiology · 2018Article
- Integrated metabolic models for xenobiotic induced mitochondrial toxicity in skeletal muscle.Redox biology · 2018Article
- YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus.Scientific reports · 2017Article
- Article
- Transcriptional reprogramming and phenotypic switching associated with the adaptation of Lactobacillus plantarum C2 to plant niches.Scientific reports · 2016Article
- Bioactive Molecules Released in Food by Lactic Acid Bacteria: Encrypted Peptides and Biogenic Amines.Frontiers in microbiology · 2016Review
- Effect of culturing conditions on the expression of key enzymes in the proteolytic system of Lactobacillus bulgaricus.Journal of Zhejiang University. Science. B · 2015Article
- Genetic and physiological responses of Bifidobacterium animalis subsp. lactis to hydrogen peroxide stress.Journal of bacteriology · 2013Article
- Lactobacillus helveticus: the proteolytic system.Frontiers in microbiology · 2013Article
- Article
- Integration of metabolic reactions and gene regulation.Molecular biotechnology · 2011Review
- Simultaneous presence of PrtH and PrtH2 proteinases in Lactobacillus helveticus Strains improves breakdown of the pure alphas1-casein.Applied and environmental microbiology · 2011Article
- Physiological and transcriptional response of Lactobacillus casei ATCC 334 to acid stress.Journal of bacteriology · 2010Article
- Article
- Development and validation of a species-independent functional gene microarray that targets lactic acid bacteria.Applied and environmental microbiology · 2009Article
- Genome sequence and comparative genome analysis of Lactobacillus casei: insights into their niche-associated evolution.Genome biology and evolution · 2009Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactobacillus helveticus CNRZ32 is used by the dairy industry to modulate cheese flavor. The compilation of a draft genome sequence for this strain allowed us to identify and completely sequence 168 genes potentially important for the growth of this organism in milk or for cheese flavor development. The primary aim of this study was to investigate the expression of these genes during growth in milk and MRS medium by using microarrays. Oligonucleotide probes against each of the completely sequenced genes were compiled on maskless photolithography-based DNA microarrays. Additionally, the entire draft genome sequence was used to produce tiled microarrays in which noninterrupted sequence contigs were covered by consecutive 24-mer probes and associated mismatch probe sets. Total RNA isolated from cells grown in skim milk or in MRS to mid-log phase was used as a template to synthesize cDNA, followed by Cy3 labeling and hybridization. An analysis of data from annotated gene probes identified 42 genes that were upregulated during the growth of CNRZ32 in milk (P < 0.05), and 25 of these genes showed upregulation after applying Bonferroni's adjustment. The tiled microarrays identified numerous additional genes that were upregulated in milk versus MRS. Collectively, array data showed the growth of CNRZ32 in milk-induced genes encoding cell-envelope proteinases, oligopeptide transporters, and endopeptidases as well as enzymes for lactose and cysteine pathways, de novo synthesis, and/or salvage pathways for purines and pyrimidines and other functions. Genes for a hypothetical phosphoserine utilization pathway were also differentially expressed. Preliminary experiments indicate that cheese-derived, phosphoserine-containing peptides increase growth rates of CNRZ32 in a chemically defined medium. These results suggest that phosphoserine is used as an energy source during the growth of L. helveticus CNRZ32.
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