ArticleMicrobiology (Reading, England)2007
Correlation between transcript profiles and fitness of deletion mutants in anaerobic chemostat cultures of Saccharomyces cerevisiae.
Article in Microbiology (Reading, England), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Transcriptomics is essential but not sufficient to unravel complex plant-pathogen interactions.Plant cell reports · 2024Article
- New Genes Involved in Osmotic Stress Tolerance inFrontiers in microbiology · 2016Article
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- Genome-wide study of the adaptation of Saccharomyces cerevisiae to the early stages of wine fermentation.PloS one · 2013Article
- Genome-wide analytical approaches for reverse metabolic engineering of industrially relevant phenotypes in yeast.FEMS yeast research · 2012Review
- Evolutionary and Biochemical Aspects of Chemical Stress Resistance in Saccharomyces cerevisiae.Frontiers in genetics · 2012Article
- Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: transcriptome analysis of anaerobic retentostat cultures.FEMS yeast research · 2011Article
- Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris.Microbial cell factories · 2011Article
- Multiple means to the same end: the genetic basis of acquired stress resistance in yeast.PLoS genetics · 2011Article
- Yeast cells can access distinct quiescent states.Genes & development · 2011Article
- Robustness and evolvability in natural chemical resistance: identification of novel systems properties, biochemical mechanisms and regulatory interactions.Molecular bioSystems · 2010Article
- Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae.BMC molecular biology · 2010Article
- Exploring and dissecting genome-wide gene expression responses of Penicillium chrysogenum to phenylacetic acid consumption and penicillinG production.BMC genomics · 2009Article
- Identification of molecular pathways affected by pterostilbene, a natural dimethylether analog of resveratrol.BMC medical genomics · 2008Article
- Article
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7 authors.
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Abstract
The applicability of transcriptomics for functional genome analysis rests on the assumption that global information on gene function can be inferred from transcriptional regulation patterns. This study investigated whether Saccharomyces cerevisiae genes that show a consistently higher transcript level under anaerobic than aerobic conditions do indeed contribute to fitness in the absence of oxygen. Tagged deletion mutants were constructed in 27 S. cerevisiae genes that showed a strong and consistent transcriptional upregulation under anaerobic conditions, irrespective of the nature of the growth-limiting nutrient (glucose, ammonia, sulfate or phosphate). Competitive anaerobic chemostat cultivation showed that only five out of the 27 mutants (eug1Delta, izh2Delta, plb2Delta, ylr413wDelta and yor012wDelta) conferred a significant disadvantage relative to a tagged reference strain. The implications of this study are that: (i) transcriptome analysis has a very limited predictive value for the contribution of individual genes to fitness under specific environmental conditions, and (ii) competitive chemostat cultivation of tagged deletion strains offers an efficient approach to select relevant leads for functional analysis studies.
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