ArticleOtolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery2007
Functional expression and microdomain localization of prestin in cultured cells.
Article in Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Enhancing prestin oligomerization for superior sonogenetics.Theranostics · 2026Article
- Abnormal Cholesterol Metabolism and Lysosomal Dysfunction Induce Age-Related Hearing Loss by Inhibiting mTORC1-TFEB-Dependent Autophagy.International journal of molecular sciences · 2023Article
- Membrane prestin expression correlates with the magnitude of prestin-associated charge movement.Hearing research · 2016Article
- Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent.Scientific reports · 2016Article
- Outer Hair Cell Lateral Wall Structure Constrains the Mobility of Plasma Membrane Proteins.PLoS genetics · 2015Article
- Localization and proteomic characterization of cholesterol-rich membrane microdomains in the inner ear.Journal of proteomics · 2014Article
- Effects of cholesterol alterations are mediated via G-protein-related pathways in outer hair cells.Pflugers Archiv : European journal of physiology · 2013Article
- Real time measures of prestin charge and fluorescence during plasma membrane trafficking reveal sub-tetrameric activity.PloS one · 2013Article
- Membrane cholesterol strongly influences confined diffusion of prestin.Biophysical journal · 2012Article
- Membrane cholesterol modulates cochlear electromechanics.Pflugers Archiv : European journal of physiology · 2011Article
- Plasticity in membrane cholesterol contributes toward electrical maturation of hearing.The Journal of biological chemistry · 2011Article
- Physiopathological function of hematoside (GM3 ganglioside).Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2011Review
- A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events.American journal of physiology. Cell physiology · 2010Article
- Glycosylation regulates prestin cellular activity.Journal of the Association for Research in Otolaryngology : JARO · 2010Article
- Cysteine mutagenesis reveals transmembrane residues associated with charge translocation in prestin.The Journal of biological chemistry · 2010Article
- Lipid lateral mobility in cochlear outer hair cells: regional differences and regulation by cholesterol.Journal of the Association for Research in Otolaryngology : JARO · 2009Article
- Immune atomic force microscopy of prestin-transfected CHO cells using quantum dots.Pflugers Archiv : European journal of physiology · 2009Article
- Amphipath-induced nanoscale changes in outer hair cell plasma membrane curvature.Biophysical journal · 2009Article
- Functional prestin transduction of immature outer hair cells from normal and prestin-null mice.Journal of the Association for Research in Otolaryngology : JARO · 2008Article
- Membrane composition modulates prestin-associated charge movement.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
introductionPrestin is an essential component of the molecular motor of cochlear outer hair cells that contribute to frequency selectivity and sensitivity of mammalian hearing. A model system to study prestin employs its transfection into cultured HEK 293 cells. Our goal was to characterize prestin's trafficking pathway and localization in the plasma membrane.
methodsWe used immuno-colocalization of prestin with intracellular and plasma membrane markers and sucrose density fractionation to analyze prestin in membrane compartments. Voltage clamping was used to measure nonlinear capacitance (NLC), prestin's electrical signature. RESULTS & DISCUSSION: Prestin targets to the membrane by 24 hours post-transfection when NLC is measurable. Prestin then concentrates into membrane foci that colocalize and fractionate with membrane microdomains. Depleting membrane cholesterol content altered prestin localization and NLC.
conclusionPrestin activity in HEK 293 cells results from expression in the plasma membrane and altering membrane lipid content affects prestin localization and activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.