ArticleBrain research2007
Extracellular glutamine is a critical modulator for regulatory volume increase in human glioma cells.
Article in Brain research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Precancer exercise capacity and metabolism during tumor development coordinate the skeletal muscle-tumor metabolic competition.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ion Channels in Glioma Malignancy.Reviews of physiology, biochemistry and pharmacology · 2021Review
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- Ammonia, like K(+), stimulates the Na(+), K(+), 2 Cl(-) cotransporter NKCC1 and the Na(+),K(+)-ATPase and interacts with endogenous ouabain in astrocytes.Neurochemical research · 2015Review
- Cortical GABAergic excitation contributes to epileptic activities around human glioma.Science translational medicine · 2014Article
- Molecular physiology of SPAK and OSR1: two Ste20-related protein kinases regulating ion transport.Physiological reviews · 2012Review
- Hydrodynamic cellular volume changes enable glioma cell invasion.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- With-No-Lysine Kinase 3 (WNK3) stimulates glioma invasion by regulating cell volume.American journal of physiology. Cell physiology · 2011Article
- siRNA-mediated inhibition of Na(+)-K(+)-2Cl- cotransporter (NKCC1) and regulatory volume increase in the chondrocyte cell line C-20/A4.The Journal of membrane biology · 2011Article
- Inhibition of the Sodium-Potassium-Chloride Cotransporter Isoform-1 reduces glioma invasion.Cancer research · 2010Article
- alpha-ENaC is a functional element of the hypertonicity-induced cation channel in HepG2 cells and it mediates proliferation.Pflugers Archiv : European journal of physiology · 2009Article
- Sodium-dependent activity of aquaporin-1 in rat glioma cells: a new mechanism of cell volume regulation.Pflugers Archiv : European journal of physiology · 2009Article
- Chloride accumulation drives volume dynamics underlying cell proliferation and migration.Journal of neurophysiology · 2009Article
- Cytoplasmic condensation is both necessary and sufficient to induce apoptotic cell death.Journal of cell science · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian cells regulate their volume to prevent unintentional changes in intracellular signaling, cell metabolism, and DNA integrity. Intentional cell volume changes occur as cells undergo proliferation, apoptosis, or cell migration. To regulate cell volume, cells use channels and transport systems to flux osmolytes across the plasma membrane followed by the obligatory movement of water. While essentially all cells are capable of regulatory volume decrease (RVD), regulatory volume increase (RVI) mechanisms have only been reported in some cell types. In this investigation, we used human glioma cells as a model system to determine conditions necessary for RVI. When exposed to hyperosmotic conditions through the addition of 30 mosM NaCl or sucrose, D54-MG and U251 glioma cell lines and glioma cells from acute patient biopsies shrunk transiently but were able to fully recover their original cell volume within 40-70 min. This ability was highly temperature sensitive and absolutely required the presence of low millimolar concentrations of l-glutamine in the extracellular solution. Other known substrates of glutamine transporters such as methyl-amino isobutyric acid (MeAIB), alanine, and threonine were unable to support RVI. The ability of cells to undergo RVI also required the presence of Na+, K+, and Cl- and was inhibited by the NKCC inhibitor, bumetanide, consistent with the involvement of a Na+/K+/2Cl- cotransporter (NKCC). Moreover, the expression of NKCC1 was demonstrated by Western blot. We concluded that regulatory volume increase in human glioma cells occurs through the uptake of Na+, K+, and Cl- by NKCC1 and is modulated by the presence of glutamine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.