ArticleJournal of thrombosis and haemostasis : JTH2007
A major role for Scar/WAVE-1 downstream of GPVI in platelets.
Article in Journal of thrombosis and haemostasis : JTH, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Review
- New insights into cytoskeletal remodeling during platelet production.Journal of thrombosis and haemostasis : JTH · 2019Review
- Integrin αBlood · 2017Review
- Deletion of the Arp2/3 complex in megakaryocytes leads to microthrombocytopenia in mice.Blood advances · 2017Article
- Prostacyclin reverses platelet stress fibre formation causing platelet aggregate instability.Scientific reports · 2017Article
- The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation.Platelets · 2017Article
- Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease.Nature communications · 2017Article
- Pdlim7 Regulates Arf6-Dependent Actin Dynamics and Is Required for Platelet-Mediated Thrombosis in Mice.PloS one · 2016Article
- Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex.Nature communications · 2015Article
- Histone deacetylase 6-mediated deacetylation of α-tubulin coordinates cytoskeletal and signaling events during platelet activation.American journal of physiology. Cell physiology · 2013Article
- Wiskott-Aldrich syndrome protein (WASp) controls the delivery of platelet transforming growth factor-β1.The Journal of biological chemistry · 2013Article
- p21 activated kinase signaling coordinates glycoprotein receptor VI-mediated platelet aggregation, lamellipodia formation, and aggregate stability under shear.Arteriosclerosis, thrombosis, and vascular biology · 2013Article
- Rho GTPases in platelet function.Journal of thrombosis and haemostasis : JTH · 2013Review
- Arp2/3 complex activity in filopodia of spreading cells.BMC cell biology · 2008Article
- Differential roles of the PKC novel isoforms, PKCdelta and PKCepsilon, in mouse and human platelets.PloS one · 2008Article
- Studies on the actin-binding protein HS1 in platelets.BMC cell biology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundThe small GTPase Rac1 plays a critical role in lamellipodia assembly in platelets on matrix proteins in the absence or presence of G protein-coupled receptor (GPCR) agonists. Rac mediates actin assembly via Scar/WAVE, a family of scaffolding proteins that direct actin reorganization by relaying signals from Rac to the Arp2/3 complex.
objectiveTo evaluate the role of Scar/WAVE-1 in mediating platelet activation and cytoskeletal reorganization. METHODS AND
resultsUsing specific antibodies, we demonstrate that murine platelets, like human platelets, express Scar/WAVE-1 and Scar/WAVE-2. Lamellipodia formation in Scar/WAVE-1(-/-) platelets is markedly inhibited on immobilized collagen-related peptide (CRP) and on laminin, both of which signal through the collagen receptor GPVI. In contrast, lamellipodia formation on collagen, which requires release of the GPCR agonists ADP and thromboxane A(2), is not altered. Immobilized fibrinogen supports limited formation of lamellipodia in murine platelets, which is not altered in Scar/WAVE-1(-/-) platelets. As with Rac1(-/-) platelets, Scar/WAVE-1(-/-) platelets exhibit a marked inhibition of aggregation in response to CRP, whereas the response to the GPCR agonist thrombin is not altered. Platelet aggregation on immobilized collagen under shear, which is dependent on signaling by matrix and GPCR agonists, was unaltered in the absence of Scar/WAVE-1.
conclusionThis study demonstrates a major role for Scar/WAVE-1 in mediating platelet cytoskeletal reorganization and aggregate formation downstream of activation by GPVI but not by GPCR agonists.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.