ArticlePlant molecular biology1992
In vivo analysis of plant RNA structure: soybean 18S ribosomal and ribulose-1,5-bisphosphate carboxylase small subunit RNAs.
Article in Plant molecular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed.
- Rock, scissors, paper: How RNA structure informs function.The Plant cell · 2023Article
- Technique Development for Probing RNA Structure In Vivo and Genome-Wide.Cold Spring Harbor perspectives in biology · 2018Review
- Genome-wide profiling of in vivo RNA structure at single-nucleotide resolution using structure-seq.Nature protocols · 2015Article
- In vivo genome-wide profiling of RNA secondary structure reveals novel regulatory features.Nature · 2014Article
- Evidence for the existence of the loop E motif of Potato spindle tuber viroid in vivo.Journal of virology · 2007Article
- Lead(II) as a probe for investigating RNA structure in vivo.RNA (New York, N.Y.) · 2002Article
- Small cis-acting sequences that specify secondary structures in a chloroplast mRNA are essential for RNA stability and translation.Molecular and cellular biology · 1999Article
- Degradation of the soybean ribulose-1,5-bisphosphate carboxylase small-subunit mRNA, SRS4, initiates with endonucleolytic cleavage.Molecular and cellular biology · 1995Article
- Faithful degradation of soybean rbcS mRNA in vitro.Molecular and cellular biology · 1994Article
- RNA annealing activities in HeLa nuclei.The EMBO journal · 1994Article
- RNA folding in living cells.RNA biologyReview
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A method to investigate the structure of RNA molecules within intact plant tissues has been developed. The RNA structures are analyzed using dimethyl sulfate (DMS), which modifies substituents of adenine and cytosine residues within single-stranded regions of RNA molecules. Reactive sites are identified by primer extension analysis. Using this procedure, an analysis of the secondary structure of the cytoplasmic 18S ribosomal RNA in soybean seedling leaves has been completed. DMS modification data are in good agreement with the phylogenetic structure predicted for soybean 18S rRNA. However, there are a few notable exceptions where residues thought to be involved in double-stranded regions in all 18S rRNAs are strongly modified in soybean leaf samples. These data taken together with the phylogenetic structure suggest that alternate structures may exist in vivo. The further applicability of this technique is demonstrated by comparing the modification pattern obtained in vivo to that obtained in vitro for a particular mRNA molecule encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. The results obtained are compared to a predicted minimum energy secondary structure. The data indicate that the conformation of RNA molecules within the cell may not be reflected in a structural analysis of purified mRNA molecules.
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Registered trials
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