ArticleBiochemistry2007
Efficient ligation of the Schistosoma hammerhead ribozyme.
Article in Biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Hammerhead Ribozymes: Structural Insights, Catalytic Mechanisms, and Cutting-Edge Applications in Synthetic Biology.International journal of molecular sciences · 2025Review
- Viroids: Non-Coding Circular RNAs Able to Autonomously Replicate and Infect Higher Plants.Biology · 2023Review
- Spin Labeling of Long RNAs Via Click Reaction and Enzymatic Ligation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Genome Evolution from Random Ligation of RNAs of Autocatalytic Sets.International journal of molecular sciences · 2021Article
- Biochemical analysis of cleavage and ligation activities of the pistol ribozyme fromRNA biology · 2021Article
- Small circRNAs with self-cleaving ribozymes are highly expressed in diverse metazoan transcriptomes.Nucleic acids research · 2020Article
- Article
- Classification of the nucleolytic ribozymes based upon catalytic mechanism.F1000Research · 2019Review
- Mutational Analysis ofFrontiers in microbiology · 2018Article
- The structure of a nucleolytic ribozyme that employs a catalytic metal ion.Nature chemical biology · 2017Article
- The Hammerhead Ribozyme: A Long History for a Short RNA.Molecules (Basel, Switzerland) · 2017Review
- Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae.Molecular plant pathology · 2016Review
- Ligation of RNA Oligomers by the Schistosoma mansoni Hammerhead Ribozyme in Frozen Solution.Journal of molecular evolution · 2016Article
- Bridging the gap between theory and experiment to derive a detailed understanding of hammerhead ribozyme catalysis.Progress in molecular biology and translational science · 2013Review
- A versatile cis-blocking and trans-activation strategy for ribozyme characterization.Nucleic acids research · 2013Article
- The hammerhead ribozyme: structure, catalysis, and gene regulation.Progress in molecular biology and translational science · 2013Review
- Involvement of the chloroplastic isoform of tRNA ligase in the replication of viroids belonging to the family Avsunviroidae.Journal of virology · 2012Article
- Additional roles of a peripheral loop-loop interaction in the Neurospora VS ribozyme.Nucleic acids research · 2011Article
- Structure-based search reveals hammerhead ribozymes in the human microbiome.The Journal of biological chemistry · 2011Article
- Small self-cleaving ribozymes.Cold Spring Harbor perspectives in biology · 2010Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The hammerhead ribozyme from Schistosoma mansoni is the best characterized of the natural hammerhead ribozymes. Biophysical, biochemical, and structural studies have shown that the formation of the loop-loop tertiary interaction between stems I and II alters the global folding, cleavage kinetics, and conformation of the catalytic core of this hammerhead, leading to a ribozyme that is readily cleaved under physiological conditions. This study investigates the ligation kinetics and the internal equilibrium between cleavage and ligation for the Schistosoma hammerhead. Single turnover kinetic studies on a construct where the ribozyme cleaves and ligates substrate(s) in trans showed up to 23% ligation when starting from fully cleaved products. This was achieved by an approximately 2000-fold increase in the rate of ligation compared to a minimal hammerhead without the loop-loop tertiary interaction, yielding an internal equilibrium that ranges from 2 to 3 at physiological Mg2+ ion concentrations (0.1-1 mM). Thus, the natural Schistosoma hammerhead ribozyme is almost as efficient at ligation as it is at cleavage. The results here are consistent with a model where formation of the loop-loop tertiary interaction leads to a higher population of catalytically active molecules and where formation of this tertiary interaction has a much larger effect on the ligation than the cleavage activity of the Schistosoma hammerhead ribozyme.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.