ArticleCellular & molecular biology letters2007
Improved fusion protein expression of EGFP via the mutation of both Kozak and the initial ATG codon.
Article in Cellular & molecular biology letters, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- On subcellular distribution of the zinc finger 469 protein (ZNF469) and observed discrepancy in the localization of endogenous and overexpressed ZNF469.FEBS open bio · 2025Article
- Development of a SARS-CoV-2 neutralization assay based on a pseudotyped virus using a HIV system.MedComm · 2024Article
- mCherry on Top: A Positive Read-Out Cellular Platform for Screening DMD Exon Skipping Xenopeptide-PMO Conjugates.Bioconjugate chemistry · 2023Article
- MS2 Virus-like Particles as a Versatile Peptide Presentation Platform: Insights into the Deterministic Abilities for Accommodating Heterologous Peptide Lengths.ACS synthetic biology · 2023Article
- [Establishment of a reporter system for estimating activation of human hepatic stellate cells based onBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2023Article
- Zinc fingers function cooperatively with KRAB domain for nuclear localization of KRAB-containing zinc finger proteins.PloS one · 2014Article
- Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1.Cellular and molecular life sciences : CMLS · 2013Article
- Synthetic biology: advancing the design of diverse genetic systems.Annual review of chemical and biomolecular engineering · 2013Review
- Fluorescent proteins: a cell biologist's user guide.Trends in cell biology · 2009Article
- The second wave of synthetic biology: from modules to systems.Nature reviews. Molecular cell biology · 2009Review
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6 authors.
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Abstract
Since its discovery, green fluorescence protein (GFP) has been used as a reporter in a broad range of applications, including the determination of gene expresion in diverse organisms, and subcellular protein localization. pEGFP-N1 is a eukayotic expression vector encoding EGFP, the MCS of which locates at the N terminus of EGFP. In this study, the cDNA sequence of scorpion toxin BmKK2 was inserted into the XhoI-HindIII cut of pEGFP-N1 to construct a toxin-EGFP fusion gene (named pEGFP-BmKK2). Fluorescence imaging revealed that HEK 293T cells that were transfected by pEGFP-BmKK2 emitted green fluorescence. Transcription of pEGFP-BmKK2 was confirmed by RT-PCR. However, western blotting analysis showed that the transfected HEK 293T cells expressed mostly EGFP, but little toxin-EGFP fusion protein, implying that pEGFP-N1 cannot be used as a fusion expression vector for subcellular protein localization for the BmKK2 gene. Consequently, two modified recombinant vectors (pEGFP-BmKK2-M1 and pEGFP-BmKK2-M2) were constructed based on pEGFP-BmKK2. This greatly improved the expression of toxin-EGFP fusion protein from pEGFP-BmKK2-M2.
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