ArticleNucleic acids research2007
Multiplex amplification of all coding sequences within 10 cancer genes by Gene-Collector.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Essential Genes Discovery in Microorganisms by Transposon-Directed Sequencing (Tn-Seq): Experimental Approaches, Major Goals, and Future Perspectives.International journal of molecular sciences · 2024Review
- Dealing with Pseudogenes in Molecular Diagnostics in the Next Generation Sequencing Era.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Molecular and Biochemical Techniques for Deciphering p53-MDM2 Regulatory Mechanisms.Biomolecules · 2020Review
- Clinical exome sequencing in neurogenetic and neuropsychiatric disorders.Annals of the New York Academy of Sciences · 2016Review
- Suppression Subtractive Hybridization Versus Next-Generation Sequencing in Plant Genetic Engineering: Challenges and Perspectives.Molecular biotechnology · 2015Review
- Highly rapid amplification-free and quantitative DNA imaging assay.Scientific reports · 2013Article
- Multiplex target capture with double-stranded DNA probes.Genome medicine · 2013Article
- Integrated Amplification Microarrays for Infectious Disease Diagnostics.Microarrays (Basel, Switzerland) · 2012Review
- Article
- Multiplex Chromosomal Exome Sequencing Accelerates Identification of ENU-Induced Mutations in the Mouse.G3 (Bethesda, Md.) · 2012Article
- Review of massively parallel DNA sequencing technologies.The HUGO journal · 2011Article
- Next-generation sequencing for cancer diagnostics: a practical perspective.The Clinical biochemist. Reviews · 2011Article
- Targeted sequencing of the human X chromosome exome.Genomics · 2011Article
- Exome sequencing as a tool for Mendelian disease gene discovery.Nature reviews. Genetics · 2011Review
- High-quality DNA sequence capture of 524 disease candidate genes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.The Journal of molecular diagnostics : JMD · 2011Article
- Performance of microarray and liquid based capture methods for target enrichment for massively parallel sequencing and SNP discovery.PloS one · 2011Article
- Genome-scale identification of resistance functions in Pseudomonas aeruginosa using Tn-seq.mBio · 2011Article
- Artificial genetic systems: self-avoiding DNA in PCR and multiplexed PCR.Angewandte Chemie (International ed. in English) · 2010Article
- Target-enrichment strategies for next-generation sequencing.Nature methods · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Herein we present Gene-Collector, a method for multiplex amplification of nucleic acids. The procedure has been employed to successfully amplify the coding sequence of 10 human cancer genes in one assay with uniform abundance of the final products. Amplification is initiated by a multiplex PCR in this case with 170 primer pairs. Each PCR product is then specifically circularized by ligation on a Collector probe capable of juxtapositioning only the perfectly matched cognate primer pairs. Any amplification artifacts typically associated with multiplex PCR derived from the use of many primer pairs such as false amplicons, primer-dimers etc. are not circularized and degraded by exonuclease treatment. Circular DNA molecules are then further enriched by randomly primed rolling circle replication. Amplification was successful for 90% of the targeted amplicons as seen by hybridization to a custom resequencing DNA micro-array. Real-time quantitative PCR revealed that 96% of the amplification products were all within 4-fold of the average abundance. Gene-Collector has utility for numerous applications such as high throughput resequencing, SNP analyses, and pathogen detection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.