ArticleChemistry & biology2007
Identification of a potential general acid/base in the reversible phosphoryl transfer reactions catalyzed by tyrosine recombinases: Flp H305.
Article in Chemistry & biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Expression of Flp Protein in a Baculovirus/Insect Cell System for Biotechnological Applications.The protein journal · 2017Article
- Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts InhibitFrontiers in pharmacology · 2017Article
- Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii.Archaea (Vancouver, B.C.) · 2016Article
- Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination.Nucleic acids research · 2015Article
- Single molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombination.Nucleic acids research · 2015Article
- The partitioning and copy number control systems of the selfish yeast plasmid: an optimized molecular design for stable persistence in host cells.Microbiology spectrum · 2014Review
- Arginine as a general acid catalyst in serine recombinase-mediated DNA cleavage.The Journal of biological chemistry · 2013Article
- Topoisomerases and site-specific recombinases: similarities in structure and mechanism.Critical reviews in biochemistry and molecular biology · 2010Review
- Sin resolvase catalytic activity and oligomerization state are tightly coupled.Journal of molecular biology · 2010Article
- Structural and kinetic analysis of Bacillus subtilis N-acetylglucosaminidase reveals a unique Asp-His dyad mechanism.The Journal of biological chemistry · 2010Article
- Requirements for catalysis in the Cre recombinase active site.Nucleic acids research · 2010Article
- Control of directionality in the DNA strand-exchange reaction catalysed by the tyrosine recombinase TnpI.Nucleic acids research · 2010Article
- Active site electrostatics protect genome integrity by blocking abortive hydrolysis during DNA recombination.The EMBO journal · 2009Article
- An interlocked dimer of the protelomerase TelK distorts DNA structure for the formation of hairpin telomeres.Molecular cell · 2007Article
- Kinetic isotope effects for alkaline phosphatase reactions: implications for the role of active-site metal ions in catalysis.Journal of the American Chemical Society · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Flp provides a unique opportunity to apply the tools of chemical biology to phosphoryl transfer reactions. Flp and other tyrosine recombinases catalyze site-specific DNA rearrangements via a phosphotyrosine intermediate. Unlike most related enzymes, Flp's nucleophilic tyrosine derives from a different protomer than the remainder of its active site. Because the tyrosine can be supplied exogenously, nonnatural synthetic analogs can be used. Here we examine the catalytic role of Flp's conserved H305. DNA cleavage was studied using a peptide containing either tyrosine (pKa congruent with 10) or 3-fluoro-tyrosine (pKa congruent with 8.4). Religation was studied using DNA substrates with 3'-phospho-cresol (pKa congruent with 10) or 3'-para-nitro-phenol (pKa congruent with 7.1). In both cases, the tyrosine analog with the lower pKa specifically restored the activity of an H305 mutant. These results provide experimental evidence that this conserved histidine functions as a general acid/base catalyst in tyrosine recombinases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.