ArticleMolecular biology of the cell2007
Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 197 citations in OpenAlex.
- Human finger-prick induced pluripotent stem cells facilitate the development of stem cell banking.Stem cells translational medicine · 2014Trial
- Capturing avian somatic cells using feather pulp fibroblast culture as a non-invasive approach to biobanking endangered birds.Cytotechnology · 2025Article
- Immune checkpoint inhibitors and Chimeric Antigen Receptor (CAR)-T cell therapy: Potential treatment options against Testicular Germ Cell Tumors.Frontiers in immunology · 2023Review
- UCH-L1 and UCH-L3 regulate the cancer stem cell-like properties through PI3 K/Akt signaling pathway in prostate cancer cells.Animal cells and systems · 2021Article
- Nanosecond pulsed electric fields enhance mesenchymal stem cells differentiation via DNMT1-regulated OCT4/NANOG gene expression.Stem cell research & therapy · 2020Article
- Transient inhibition of mTOR in human pluripotent stem cells enables robust formation of mouse-human chimeric embryos.Science advances · 2020Article
- Hydrogen sulfide facilitates reprogramming and trans-differentiation in 3D dermal fibroblast.PloS one · 2020Article
- Human Induced Pluripotent Stem-Cell-Derived Cardiomyocytes as Models for Genetic Cardiomyopathies.International journal of molecular sciences · 2019Review
- PIXUL-ChIP: integrated high-throughput sample preparation and analytical platform for epigenetic studies.Nucleic acids research · 2019Article
- Embryonic germ cell extracts erase imprinted genes and improve the efficiency of induced pluripotent stem cells.Scientific reports · 2018Article
- Imprinted gene Zinc finger protein 127 is a novel regulator of master pluripotency transcription factor, Oct4.BMB reports · 2018Article
- Emerging Prognostic Biomarkers in Testicular Germ Cell Tumors: Looking Beyond Established Practice.Frontiers in oncology · 2018Article
- Extract of mouse embryonic stem cells induces the expression of pluripotency genes in human adipose tissue-derived stem cells.Iranian journal of basic medical sciences · 2017Article
- DHFR and MDR1 upregulation is associated with chemoresistance in osteosarcoma stem-like cells.Oncology letters · 2017Article
- NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells.International journal of molecular sciences · 2017Article
- Enhancement of β-Globin Gene Expression in Thalassemic IVS2-654 Induced Pluripotent Stem Cell-Derived Erythroid Cells by Modified U7 snRNA.Stem cells translational medicine · 2017Article
- Cellular Reprogramming Using Protein and Cell-Penetrating Peptides.International journal of molecular sciences · 2017Review
- Mechanisms Regulating Stemness and Differentiation in Embryonal Carcinoma Cells.Stem cells international · 2017Review
- Components of chicken egg white extract smaller than 3 kDa in size promote 293T cell proliferation.Cytotechnology · 2016Article
- Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4.Stem cells international · 2016Article
23 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analyses of molecular events associated with reprogramming somatic nuclei to pluripotency are scarce. We previously reported the reprogramming of epithelial cells by extract of undifferentiated embryonal carcinoma (EC) cells. We now demonstrate reprogramming of DNA methylation and histone modifications on regulatory regions of the developmentally regulated OCT4 and NANOG genes by exposure of 293T cells to EC cell extract. OCT4 and NANOG are transcriptionally up-regulated and undergo mosaic cytosine-phosphate-guanosine demethylation. OCT4 demethylation occurs as early as week 1, is enhanced by week 2, and is most prominent in the proximal promoter and distal enhancer. Targeted OCT4 and NANOG demethylation does not occur in 293T extract-treated cells. Retinoic acid-mediated differentiation of reprogrammed cells elicits OCT4 promoter remethylation and transcriptional repression. Chromatin immunoprecipitation analyses of lysines K4, K9, and K27 of histone H3 on OCT4 and NANOG indicate that primary chromatin remodeling determinants are acetylation of H3K9 and demethylation of dimethylated H3K9. H3K4 remains di- and trimethylated. Demethylation of trimethylated H3K9 and H3K27 also occurs; however, trimethylation seems more stable than dimethylation. We conclude that a central epigenetic reprogramming event is relaxation of chromatin at loci associated with pluripotency to create a conformation compatible with transcriptional activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.