ArticleJournal of virology2007
Determination of the ex vivo rates of human immunodeficiency virus type 1 reverse transcription by using novel strand-specific amplification analysis.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- HIV-1 uncoating requires long double-stranded reverse transcription products.Science advances · 2024Article
- Mathematical Modelling of the Molecular Mechanisms of Interaction of Tenofovir with Emtricitabine against HIV.Viruses · 2021Article
- HIV-1 uncoating occurs via a series of rapid biomechanical changes in the core related to individual stages of reverse transcription.Journal of virology · 2021Article
- Recombination is required for efficient HIV-1 replication and the maintenance of viral genome integrity.Nucleic acids research · 2018Article
- Clinical use of lentiviral vectors.Leukemia · 2018Review
- Reverse Transcription of Retroviruses and LTR Retrotransposons.Microbiology spectrum · 2015Review
- A cell-based strategy to assess intrinsic inhibition efficiencies of HIV-1 reverse transcriptase inhibitors.Antimicrobial agents and chemotherapy · 2015Article
- Article
- Nuclear import of APOBEC3F-labeled HIV-1 preintegration complexes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The importance of becoming double-stranded: Innate immunity and the kinetic model of HIV-1 central plus strand synthesis.Virology · 2013Review
- Delaying reverse transcription does not increase sensitivity of HIV-1 to human TRIM5α.PloS one · 2013Article
- HIV-1 reverse transcription.Cold Spring Harbor perspectives in medicine · 2012Review
- HIV-1 polymerase inhibition by nucleoside analogs: cellular- and kinetic parameters of efficacy, susceptibility and resistance selection.PLoS computational biology · 2012Article
- Strain-specific differences in the impact of human TRIM5alpha, different TRIM5alpha alleles, and the inhibition of capsid-cyclophilin A interactions on the infectivity of HIV-1.Journal of virology · 2010Article
- APOBEC3F and APOBEC3G inhibit HIV-1 DNA integration by different mechanisms.Journal of virology · 2010Article
- Reverse Transcriptase and Cellular Factors: Regulators of HIV-1 Reverse Transcription.Viruses · 2009Article
- The remarkable frequency of human immunodeficiency virus type 1 genetic recombination.Microbiology and molecular biology reviews : MMBR · 2009Review
- Nucleocapsid protein function in early infection processes.Virus research · 2008Review
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Replication of human immunodeficiency virus type 1 (HIV-1), like all organisms, involves synthesis of a minus-strand and a plus-strand of nucleic acid. Currently available PCR methods cannot distinguish between the two strands of nucleic acids. To carry out detailed analysis of HIV-1 reverse transcription from infected cells, we have developed a novel strand-specific amplification (SSA) assay using single-stranded padlock probes that are specifically hybridized to a target strand, ligated, and quantified for sensitive analysis of the kinetics of HIV-1 reverse transcription in cells. Using SSA, we have determined for the first time the ex vivo rates of HIV-1 minus-strand DNA synthesis in 293T and human primary CD4(+) T cells ( approximately 68 to 70 nucleotides/min). We also determined the rates of minus-strand DNA transfer ( approximately 4 min), plus-strand DNA transfer ( approximately 26 min), and initiation of plus-strand DNA synthesis ( approximately 9 min) in 293T cells. Additionally, our results indicate that plus-strand DNA synthesis is initiated at multiple sites and that several reverse transcriptase inhibitors influence the kinetics of minus-strand DNA synthesis differently, providing insights into their mechanism of inhibition. The SSA technology provides a novel approach to analyzing DNA replication processes and should facilitate the development of new antiretroviral drugs that target specific steps in HIV-1 reverse transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.