ArticleJournal of cell science2007
Differential dynamics of Rab3A and Rab27A on secretory granules.
Article in Journal of cell science, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 65 citations in OpenAlex.
- Multiple pathways and independent functional pools in insulin granule exocytosis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Review
- Article
- In vivo Roles of Rab27 and Its Effectors in Exocytosis.Cell structure and function · 2021Review
- Endosomal Rab GTPases regulate secretory granule maturation inCommunicative & integrative biology · 2021Article
- Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.Physiological reports · 2020Article
- Article
- Membrane trafficking in osteoclasts and implications for osteoporosis.Biochemical Society transactions · 2019Review
- The adaptor protein melanophilin regulates dynamic myosin-Va:cargo interaction and dendrite development in melanocytes.Molecular biology of the cell · 2019Article
- Epistatic Analysis of the Contribution of Rabs and Kifs to CATCHR Family Dependent Golgi Organization.Frontiers in cell and developmental biology · 2019Article
- Infection-Induced Changes Within the Endocytic Recycling Compartment Suggest a Roadmap of Human Cytomegalovirus Egress.Frontiers in microbiology · 2018Article
- Imbalanced Rab3D versus Rab27 increases cathepsin S secretion from lacrimal acini in a mouse model of Sjögren's Syndrome.American journal of physiology. Cell physiology · 2016Article
- Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.Histochemistry and cell biology · 2016Article
- Functions of Rab Proteins at Presynaptic Sites.Cells · 2016Review
- Warburg Micro syndrome is caused by RAB18 deficiency or dysregulation.Open biology · 2015Article
- Distinct actions of Rab3 and Rab27 GTPases on late stages of exocytosis of insulin.Traffic (Copenhagen, Denmark) · 2014Article
- Late steps in secretory lysosome exocytosis in cytotoxic lymphocytes.Frontiers in immunology · 2013Review
- A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells.Molecular biology of the cell · 2013Article
- Article
- Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have assessed the dynamics of the association of Rab3A and Rab27A with secretory granules at various stages of their life in PC12 cells. Endogenous Rab3A colocalised with the secretory granule marker secretogranin II (SGII) and expressed EGFP-Rab3A and ECFP-Rab27A colocalised with one another. The extent of colocalisation between EGFP-Rab3A or EGFP-Rab27 and SGII increased after longer times post transfection suggesting that these Rab proteins are preferentially recruited to newly synthesised granules. Following the release of immature secretory granules from the trans-Golgi network, Rab3A and Rab27A became associated with the immature granules after a lag period of around 20 minutes. Rab dynamics on granules were analysed in fluorescence recovery after photobleaching (FRAP) experiments. The recovery profile of EGFP-Rab27A was comparable to that of ppANF-EGFP, whereas the recovery profile of EGFP-Rab3A was significantly faster, indicating that Rab3A but not Rab27A might be rapidly exchanged between granules and cytosol. Inhibition of heat-shock protein 90 with 10 muM geldanamycin did not affect the exchange process or regulated exocytosis. Rab dynamics during stimulation with 300 muM ATP were analysed in live cells. Loss of granular ppANF-EGFP fluorescence was seen at the cell periphery after stimulation but only limited changes in EGFP-Rab3A and EGFP-Rab27A fluorescence was observed, indicating that the Rab proteins do not immediately dissociate or disperse on stimulation. The data suggest potentially distinct roles for Rab3A and Rab27A and we suggest that the finding that young secretory granules have a higher capacity for binding Rab3A and Rab27A is functionally important for preferential exocytosis from these granules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.