ArticlePloS one2007
PathogenMip assay: a multiplex pathogen detection assay.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Molecular approaches to recognize relevant and emerging infectious diseases in animals.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Microfluidic quantitative PCR for simultaneous quantification of multiple viruses in environmental water samples.Applied and environmental microbiology · 2014Article
- Molecular inversion probe: a new tool for highly specific detection of plant pathogens.PloS one · 2014Article
- Simultaneous quantification of multiple food- and waterborne pathogens by use of microfluidic quantitative PCR.Applied and environmental microbiology · 2013Article
- Article
- A rapid, cost-effective method of assembly and purification of synthetic DNA probes >100 bp.PloS one · 2012Article
- Article
- Application of hybridization control probe to increase accuracy on ligation detection or minisequencing diagnostic microarrays.BMC research notes · 2009Article
- Basic concepts of microarrays and potential applications in clinical microbiology.Clinical microbiology reviews · 2009Review
- The relationship between human papillomavirus status and other molecular prognostic markers in head and neck squamous cell carcinomas.International journal of radiation oncology, biology, physics · 2009Article
- Nested Patch PCR enables highly multiplexed mutation discovery in candidate genes.Genome research · 2008Article
- Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications.PloS one · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonstrated the utility of this assay with an initial set of 24 probes targeting the most clinically relevant HPV genotypes associated with cervical cancer progression. Probe construction was based on a novel, cost-effective, ligase-based protocol. The assay was validated by performing pyrosequencing and Microarray chip detection in parallel experiments. HPV plasmids were used to validate sensitivity and selectivity of the assay. In addition, 20 genomic DNA extracts from primary tumors were genotyped with the PathogenMip Assay results and were in 100% agreement with conventional sequencing using an L1-based HPV genotyping protocol. The PathogenMip Assay is a widely accessible protocol for producing and using highly discriminating probes, with experimentally validated results in pathogen genotyping, which could potentially be applied to the detection and characterization of any microbe.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.