ArticleJournal of the American Chemical Society2007
Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.
Article in Journal of the American Chemical Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 74 citations in OpenAlex.
- The deacetylase Sir2 is the primary sensor driving transcriptional changes in response to low NAD+ in the yeast Kluyveromyces lactis.Genetics · 2026Article
- ZmTH1 Is Vital for Healthy Plant Growth and Promotes Cold/Drought Tolerance by Regulating Thiamin Diphosphate-Dependent Metabolisms in Maize.Plant biotechnology journal · 2026Article
- Comparative Proteomic Analysis ofJournal of fungi (Basel, Switzerland) · 2025Article
- From Synthesis to Solutions: Hydrogels' Impact on the Biomedical Landscape.Current pharmaceutical biotechnology · 2025Review
- Article
- The involvement of effector memory CD4Frontiers in aging neuroscience · 2024Article
- Hydrogels-A Promising Materials for 3D Printing Technology.Gels (Basel, Switzerland) · 2023Review
- Oxidative Dearomatization of PLP in Thiamin Pyrimidine Biosynthesis inJournal of the American Chemical Society · 2023Article
- Adaptive Laboratory Evolution and Reverse Engineering of Single-Vitamin Prototrophies in Saccharomyces cerevisiae.Applied and environmental microbiology · 2020Article
- Vitamin requirements and biosynthesis in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2020Review
- Ascorbate and Thiamin: Metabolic Modulators in Plant Acclimation Responses.Plants (Basel, Switzerland) · 2020Review
- The Effect of Oxidative Stress Towards The Expression of Thiamine Biosynthesis Genes (THIC and THI1/THI4) in Oil Palm (Tropical life sciences research · 2018Article
- Temporal Proteomics of Inducible RNAi Lines of Clp Protease Subunits Identifies Putative Protease Substrates.Plant physiology · 2018Article
- Overexpression of THI4 and HAP4 Improves Glucose Metabolism and Ethanol Production inFrontiers in microbiology · 2018Article
- Vitamin B1 diversity and characterization of biosynthesis genes in cassava.Journal of experimental botany · 2017Article
- Leveraging Genetic-Background Effects in Saccharomyces cerevisiae To Improve Lignocellulosic Hydrolysate Tolerance.Applied and environmental microbiology · 2016Article
- From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis.Journal of the American Chemical Society · 2016Article
- THI1, a Thiamine Thiazole Synthase, Interacts with Ca2+-Dependent Protein Kinase CPK33 and Modulates the S-Type Anion Channels and Stomatal Closure in Arabidopsis.Plant physiology · 2016Article
- Overexpression of Thiamin Biosynthesis Genes in Rice Increases Leaf and Unpolished Grain Thiamin Content But Not Resistance to Xanthomonas oryzae pv. oryzae.Frontiers in plant science · 2016Article
- Overexpression of a Water-Forming NADH Oxidase Improves the Metabolism and Stress Tolerance of Saccharomyces cerevisiae in Aerobic Fermentation.Frontiers in microbiology · 2016Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
Thiazole synthase catalyzes the formation of the thiazole moiety of thiamin pyrophosphate. The enzyme from Saccharomyces cerevisiae (THI4) copurifies with a set of strongly bound adenylated metabolites. One of them has been characterized as the ADP adduct of 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid. Attempts toward yielding active wild-type THI4 by releasing protein-bound metabolites have failed so far. Here, we describe the identification and characterization of two partially active mutants (C204A and H200N) of THI4. Both mutants catalyzed the release of the nicotinamide moiety from NAD to produce ADP-ribose, which was further converted to ADP-ribulose. In the presence of glycine, both the mutants catalyzed the formation of an advanced intermediate. The intermediate was trapped with ortho-phenylenediamine, yielding a stable quinoxaline derivative, which was characterized by NMR spectroscopy and ESI-MS. These observations confirm NAD as the substrate for THI4 and elucidate the early steps of this unique biosynthesis of the thiazole moiety of thiamin in eukaryotes.
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