ArticleMarine biotechnology (New York, N.Y.)
Molecular cloning and expression analysis of the myostatin gene in sea perch (Lateolabrax japonicus).
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 40 citations in OpenAlex.
- Enhanced Muscle Fibers ofInternational journal of molecular sciences · 2022Article
- Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System.Scientific reports · 2017Article
- The effects of exogenous cortisol on myostatin transcription in rainbow trout, Oncorhynchus mykiss.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2014Article
- Organization and functional analysis of the 5' flanking regions of myostatin-1 and 2 genes from Larimichthys crocea.DNA and cell biology · 2012Article
- Both WFIKKN1 and WFIKKN2 have high affinity for growth and differentiation factors 8 and 11.The Journal of biological chemistry · 2008Article
- Molecular characterization, tissue expression and sequence variability of the barramundi (Lates calcarifer) myostatin gene.BMC genomics · 2008Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myostatin (MSTN) is a member of the transforming growth factor-beta (TGF-beta) superfamily that functions as a negative regulator of skeletal muscle development and growth in mammals. However, few reports are available about the structure and function of MSTN in teleost. Here, the MSTN gene was cloned from sea perch (Lateolabrax japonicus) by homology cloning and genomic walking. In the 4873-bp genomic sequence, three exons, two introns, and 5' and 3' flanking sequences were identified. The sea perch MSTN gene encodes a 374-amino acid protein, including a signal peptide, conserved cysteine residues, and a RXXR proteolytic cleavage domain. Expression analysis of MSTN revealed that MSTN was highly expressed in eyes, brain, and muscle; intermediately in intestine; and weakly in gill, spleen, liver, and heart. It was demonstrated that MSTN mRNA was highly expressed in embryonic stem cell line (LJES1), but it was undetectable in several types of somatic cell lines from sea perch, including fibroblast-like cell, epithelioid cell, and lymphocyte-like cell. Further, it was demonstrated that the 5' flanking region of the MSTN gene can drive the expression of green fluorescent protein (GFP) reporter gene in LJES1 cells and transgenic zebrafish (Danio rerio). This is the first report on the expression profile of MSTN gene in various types of cell cultures.
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