ArticleVeterinary research communications2007
Diagnosis of canine brucellosis: comparison between serological and microbiological tests and a PCR based on primers to 16S-23S rDNA interspacer.
Article in Veterinary research communications, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 80 citations in OpenAlex.
- Brucellosis Abattoir Surveillance Using Serology and Molecular Tests Among Livestock in Free State and Limpopo Provinces, South Africa.Microorganisms · 2026Article
- First isolation and molecular confirmation of Brucella canis in dogs from Egypt.BMC microbiology · 2026Article
- One Health approach for Brucella canis: Serological and molecular detection in animal-hoarding individuals and their dogs.PLoS neglected tropical diseases · 2024Article
- The emergence ofEmerging microbes & infections · 2023Review
- Evaluation of Three Serological Tests for Diagnosis of Canine Brucellosis.Microorganisms · 2023Article
- Seroprevalence, and molecular detection ofInfection ecology & epidemiology · 2023Article
- Chronic Immune-Mediated Orchitis Is the Major Cause of Acquired Non-obstructive Azoospermia in Dogs.Frontiers in veterinary science · 2022Article
- Comparison of BP26, Omp25 and Omp31 and a Multiepitope-Based Fusion Protein in the Serological Detection of Canine Brucellosis.Infection and drug resistance · 2022Article
- Importance of brucellosis control programs of livestock on the improvement of one health.The veterinary quarterly · 2021Review
- Canine Brucellosis: An Update.Frontiers in veterinary science · 2021Review
- Development of a Genus-SpecificVeterinary sciences · 2020Article
- Diagnosis of canine brucellosis: comparison of various serologic tests and PCR.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Seroprevalence and characterization of Brucella species in cattle slaughtered at Gauteng abattoirs, South Africa.Veterinary medicine and science · 2019Article
- In silico design and in vitro development of a highly accurate test to detect Brucella speciesTurkish journal of medical sciences · 2019Article
- Canine Brucellosis: Insights Into the Epidemiologic Situation in Europe.Frontiers in veterinary science · 2019Article
- Brucellosis in Dogs and Public Health Risk.Emerging infectious diseases · 2018Article
- Comparison of four polymerase chain reaction assays for the detection ofVeterinary world · 2018Article
- Review
- Characterization and evaluation of an arbitrary primed Polymerase Chain Reaction (PCR) product for the specific detection of Brucella species.Saudi journal of biological sciences · 2015Article
- Isolation of Actinobacillus seminis from a goat with clinical epididymo-orchitis in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A pair of primers directed to 16S-23S rDNA interspacer (ITS) was designed directed to Brucella genetic sequences in order to develop a polymerase chain reaction (PCR) putatively capable of amplifying DNA from any Brucella species. Nucleic acid extracts from whole-blood from naive dogs were spiked with decreasing amounts of Brucella canis RM6/66 DNA and the resulting solutions were tested by PCR. In addition, the ability of PCR to amplify Brucella spp. genetic sequences from naturally infected dogs was evaluated using 210 whole-blood samples of dogs from 19 kennels. The whole-blood samples collected were subjected to blood culture and PCR. Serodiagnosis was performed using the rapid slide agglutination test with and without 2-mercaptoethanol. The DNA from whole blood was extracted using proteinase-K, sodium dodecyl sulphate and cetyl trimethyl ammonium bromide followed by phenol-chloroform purification. The PCR was capable of detecting as little as 3.8 fg of Brucella DNA mixed with 450 ng of host DNA. Theoretically, 3.8 fg of Brucella DNA represents the total genomic mass of fewer than two bacterial cells. The PCR diagnostic sensitivity and specificity were 100%. From the results observed in the present study, we conclude that PCR could be used as confirmatory test for diagnosis of B. canis infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.