ArticleNucleic acids research2007
The availability of the primer activation signal (PAS) affects the efficiency of HIV-1 reverse transcription initiation.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Retroviral PBS-segment sequence and structure: Orchestrating early and late replication events.Retrovirology · 2024Review
- Identification of a putative Gag binding site critical for feline immunodeficiency virus genomic RNA packaging.RNA (New York, N.Y.) · 2023Article
- The three-way junction structure of the HIV-1 PBS-segment binds host enzyme important for viral infectivity.Nucleic acids research · 2021Article
- Extended Interactions between HIV-1 Viral RNA and tRNAInternational journal of molecular sciences · 2020Article
- Distinct Conformational States Underlie Pausing during Initiation of HIV-1 Reverse Transcription.Journal of molecular biology · 2020Article
- Relating Structure and Dynamics in RNA Biology.Cold Spring Harbor perspectives in biology · 2019Review
- Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex.Journal of molecular biology · 2018Article
- Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization.The Journal of biological chemistry · 2018Article
- Heterogeneous structures formed by conserved RNA sequences within the HIV reverse transcription initiation site.RNA (New York, N.Y.) · 2016Article
- DHX9/RHA Binding to the PBS-Segment of the Genomic RNA during HIV-1 Assembly Bolsters Virion Infectivity.Journal of molecular biology · 2016Article
- The Interaction between tRNA(Lys) 3 and the primer activation signal deciphered by NMR spectroscopy.PloS one · 2013Article
- Article
- Coordinate roles of Gag and RNA helicase A in promoting the annealing of formula to HIV-1 RNA.Journal of virology · 2011Article
- Initiation of HIV Reverse Transcription.Viruses · 2010Article
- Destabilization of the TAR hairpin affects the structure and function of the HIV-1 leader RNA.Nucleic acids research · 2008Article
- Review
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5 authors.
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Abstract
Initiation of reverse transcription of a retroviral RNA genome is strictly regulated. The tRNA primer binds to the primer binding site (PBS), and subsequent priming is triggered by the primer activation signal (PAS) that also pairs with the tRNA. We observed that in vitro reverse transcription initiation of the HIV-1 leader RNA varies in efficiency among 3'-end truncated transcripts, despite the presence of both PBS and PAS motifs. As the HIV-1 leader RNA can adopt two different foldings, we investigated if the conformational state of the transcripts did influence the efficiency of reverse transcription initiation. However, mutant transcripts that exclusively fold one or the other structure were similarly active, thereby excluding the possibility of regulation of reverse transcription initiation by the structure riboswitch. We next set out to determine the availability of the PAS element. This sequence motif enhances the efficiency of reverse transcription initiation, but its activity is regulated because the PAS motif is initially base paired within the wild-type template. We measured that the initiation efficiency on different templates correlates directly with accessibility of the PAS motif. Furthermore, changes in PAS are critical to facilitate a primer-switch to a new tRNA species, demonstrating the importance of this enhancer element.
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