ArticlePlant physiology2007
Assessing the efficiency of RNA interference for maize functional genomics.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 65 citations in OpenAlex.
- Contribution of the tobamovirus resistance gene Tm-1 to control of tomato brown rugose fruit virus (ToBRFV) resistance in tomato.PLoS genetics · 2025Article
- Functional analysis of polyphenol oxidase 1 gene in common wheat.Frontiers in plant science · 2023Article
- Genetic Engineering of Lesquerella with Increased Ricinoleic Acid Content in Seed Oil.Plants (Basel, Switzerland) · 2021Article
- Host-Induced Gene Silencing: A Powerful Strategy to Control Diseases of Wheat and Barley.International journal of molecular sciences · 2019Review
- Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize.Frontiers in plant science · 2017Article
- Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics.Current genomics · 2016Article
- Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat.BMC plant biology · 2016Article
- Down-Regulating the Expression of 53 Soybean Transcription Factor Genes Uncovers a Role for SPEECHLESS in Initiating Stomatal Cell Lineages during Embryo Development.Plant physiology · 2015Article
- Genetic and genomic toolbox of Zea mays.Genetics · 2015Review
- Genetic perturbation of the maize methylome.The Plant cell · 2014Article
- Assessment of RNAi-induced silencing in banana (Musa spp.).BMC research notes · 2014Article
- The WD40-repeat proteins NFC101 and NFC102 regulate different aspects of maize development through chromatin modification.The Plant cell · 2013Article
- Interspecific RNA interference of SHOOT MERISTEMLESS-like disrupts Cuscuta pentagona plant parasitism.The Plant cell · 2012Article
- Production of human growth hormone in transgenic rice seeds: co-introduction of RNA interference cassette for suppressing the gene expression of endogenous storage proteins.Plant cell reports · 2012Article
- Increasing the amylose content of durum wheat through silencing of the SBEIIa genes.BMC plant biology · 2010Article
- Transgene constructs lacking transcription termination signal induce efficient silencing of endogenous targets in Arabidopsis.Molecular genetics and genomics : MGG · 2009Article
- Fused sister kinetochores initiate the reductional division in meiosis I.Nature cell biology · 2009Article
- RNA silencing mediated by direct repeats in maize: a potential tool for functional genomics.Molecular biotechnology · 2009Article
- Efficient and stable transgene suppression via RNAi in field-grown poplars.Transgenic research · 2008Article
- RNA interference for wheat functional gene analysis.Transgenic research · 2007Review
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large-scale functional genomics project was initiated to study the function of chromatin-related genes in maize (Zea mays). Transgenic lines containing short gene segments in inverted repeat orientation designed to reduce expression of target genes by RNA interference (RNAi) were isolated, propagated, and analyzed in a variety of assays. Analysis of the selectable marker expression over multiple generations revealed that most transgenes were transmitted faithfully, whereas some displayed reduced transmission or transgene silencing. A range of target-gene silencing efficiencies, from nondetectable silencing to nearly complete silencing, was revealed by semiquantitative reverse transcription-PCR analysis of transcript abundance for the target gene. In some cases, the RNAi construct was able to cause a reduction in the steady-state RNA levels of not only the target gene, but also another closely related gene. Correlation of silencing efficiency with expression level of the target gene and sequence features of the inverted repeat did not reveal any factors capable of predicting the silencing success of a particular RNAi-inducing construct. The frequencies of success of this large-scale project in maize, together with parameters for optimization at various steps, should serve as a useful framework for designing future RNAi-based functional genomics projects in crop plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.