ArticleJournal of bacteriology2007
Transposon mutagenesis identifies sites upstream of the Neisseria gonorrhoeae pilE gene that modulate pilin antigenic variation.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Restriction-modification systems are required forProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Restriction-modification systems are required forbioRxiv : the preprint server for biology · 2026Article
- Article
- Complete genome sequence ofStandards in genomic sciences · 2017Article
- Antigenic Variation in Bacterial Pathogens.Microbiology spectrum · 2016Article
- Neisseria gonorrhoeae MutS affects pilin antigenic variation through mismatch correction and not by pilE guanine quartet binding.Journal of bacteriology · 2015Article
- Neisseria gonorrhoeae RecQ helicase HRDC domains are essential for efficient binding and unwinding of the pilE guanine quartet structure required for pilin antigenic variation.Journal of bacteriology · 2013Article
- Transcription of a cis-acting, noncoding, small RNA is required for pilin antigenic variation in Neisseria gonorrhoeae.PLoS pathogens · 2013Article
- Identification of bacterial protein O-oligosaccharyltransferases and their glycoprotein substrates.PloS one · 2013Article
- Microbial antigenic variation mediated by homologous DNA recombination.FEMS microbiology reviews · 2012Review
- Inhibition of IL-10 production by maternal antibodies against Group B Streptococcus GAPDH confers immunity to offspring by favoring neutrophil recruitment.PLoS pathogens · 2011Article
- Focusing homologous recombination: pilin antigenic variation in the pathogenic Neisseria.Molecular microbiology · 2011Review
- Frequency and rate of pilin antigenic variation of Neisseria meningitidis.Journal of bacteriology · 2010Article
- Pilin antigenic variation occurs independently of the RecBCD pathway in Neisseria gonorrhoeae.Journal of bacteriology · 2009Article
- An alternative DNA structure is necessary for pilin antigenic variation in Neisseria gonorrhoeae.Science (New York, N.Y.) · 2009Article
- Role for the RecBCD recombination pathway for pilE gene variation in repair-proficient Neisseria gonorrhoeae.Journal of bacteriology · 2007Article
- G-quadruplexes in bacteria: insights into the regulatory roles and interacting proteins of non-canonical nucleic acid structures.Critical reviews in biochemistry and molecular biologyReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Gene conversion mediates the variation of virulence-associated surface structures on pathogenic microorganisms, which prevents host humoral immune responses from being effective. One of the best-studied gene conversion systems is antigenic variation (Av) of the pilin subunit of the Neisseria gonorrhoeae type IV pilus. To identify cis-acting DNA sequences that facilitate Av, the 700-bp region upstream of the pilin gene pilE was targeted for transposon mutagenesis. Four classes of transposon-associated mutations were isolated, distinguishable by their pilus-associated phenotypes: (i) insertions that did not alter Av or piliation, (ii) insertions that blocked Av, (iii) insertions that interfered with Av, and (iv) insertions that interfered with pilus expression and Av. Mutagenesis of the pilE promoter did not affect the frequency of Av, directly demonstrating that pilin Av is independent of pilE transcription. Two stretches of sequence upstream of pilE were devoid of transposon insertions, and some deletions in these regions were not recoverable, suggesting that they are essential for gonococcal viability. Insertions that blocked pilin Av were located downstream of the RS1 repeat sequence, and deletion of the region surrounding these insertions completely abrogated pilin Av, confirming that specific sequences 5' to pilE are essential for the recombination events underlying pilin Av.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.