ArticleJournal of bacteriology2007
Lipolytic enzymes in Myxococcus xanthus.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- A Lipase Gene ofInternational journal of molecular sciences · 2024Article
- Cell-cell transfer of adaptation traits benefits kin and actor in a cooperative microbe.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Purification and Optimization of Extracellular Lipase from a Novel StrainInternational journal of analytical chemistry · 2022Article
- Article
- Microbial lipases and their industrial applications: a comprehensive review.Microbial cell factories · 2020Review
- Fatty Acid Oxidation Is Required for Myxococcus xanthus Development.Journal of bacteriology · 2018Article
- Realm of Thermoalkaline Lipases in Bioprocess Commodities.Journal of lipids · 2018Review
- Proteome Analyses of Soil Bacteria Grown in the Presence of Potato Suberin, a Recalcitrant Biopolymer.Microbes and environments · 2016Article
- Neutral and Phospholipids of the Myxococcus xanthus Lipodome during Fruiting Body Formation and Germination.Applied and environmental microbiology · 2015Article
- Fatty acids from membrane lipids become incorporated into lipid bodies during Myxococcus xanthus differentiation.PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of Myxococcus xanthus encodes lipolytic enzymes in three different families: patatin lipases, alpha/beta hydrolases, and GDSL lipases. One member of each family was characterized. The protein encoded by MXAN_3852 contains motifs characteristic of patatins. MXAN_5522 encodes a protein with the G-X-S-X-G motif characteristic of the lipase subfamily of alpha/beta hydrolases. MXAN_4569 encodes a member of the GDSL family of lipolytic enzymes. Strains with deletions of MXAN_5522 and MXAN_4569 undergo faster development and earlier myxospore formation than the wild-type strain. The MXAN_5522 mutation results in spore yields substantially higher than those seen for wild-type cells. Gene expression analysis using translational lacZ fusions indicates that while all three genes are expressed during development, only MXAN_5522 and MXAN_4569 are expressed during vegetative growth. The proteins encoded by these genes were overexpressed using a T7 RNA polymerase transcription (pET102/D-TOPO) system in Escherichia coli BL21 Star (DE3) cells. The substrate specificities of the purified enzymes were investigated using p-nitrophenyl esters with chain lengths from C(2) to C(16). These enzymes preferentially hydrolyzed esters of short-chain fatty acids, yielding the highest activity with p-nitrophenyl acetate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.