ArticleThe Journal of cell biology1992
GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 119 citations in OpenAlex.
- Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays.Methods in cell biology · 2014Article
- Inner/Outer nuclear membrane fusion in nuclear pore assembly: biochemical demonstration and molecular analysis.Molecular biology of the cell · 2010Article
- The nuclear envelope.Cold Spring Harbor perspectives in biology · 2010Review
- Border control at the nucleus: biogenesis and organization of the nuclear membrane and pore complexes.Developmental cell · 2009Review
- Reorganization of the nuclear envelope during open mitosis.Current opinion in cell biology · 2008Review
- Reshaping of the endoplasmic reticulum limits the rate for nuclear envelope formation.The Journal of cell biology · 2008Article
- The life cycle of the metazoan nuclear envelope.Current opinion in cell biology · 2008Review
- Transmembrane proteins are not required for early stages of nuclear envelope assembly.The Biochemical journal · 2006Article
- TPX2 is required for postmitotic nuclear assembly in cell-free Xenopus laevis egg extracts.The Journal of cell biology · 2006Article
- Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.Molecular biology of the cell · 2003Article
- Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction?Molecular and cellular biology · 2003Article
- The Ran GTPase cycle is required for yeast nuclear pore complex assembly.The Journal of cell biology · 2003Article
- Barrier-to-autointegration factor: major roles in chromatin decondensation and nuclear assembly.The Journal of cell biology · 2002Article
- Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation.The Journal of cell biology · 2002Article
- Transport into and out of the nucleus.Microbiology and molecular biology reviews : MMBR · 2001Review
- A role for nuclear lamins in nuclear envelope assembly.The Journal of cell biology · 2001Article
- In vitro formation of the endoplasmic reticulum occurs independently of microtubules by a controlled fusion reaction.The Journal of cell biology · 2000Article
- Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2beta proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts.The Journal of cell biology · 1999Article
- A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly.Molecular biology of the cell · 1998Article
- The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function.Molecular biology of the cell · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear envelope assembly was studied in vitro using extracts from Xenopus eggs. Nuclear-specific vesicles bound to demembranated sperm chromatin but did not fuse in the absence of cytosol. Addition of cytosol stimulated vesicle fusion, pore complex assembly, and eventual nuclear envelope growth. Vesicle binding and fusion were assayed by light and electron microscopy. Addition of ATP and GTP to bound vesicles caused limited vesicle fusion, but enclosure of the chromatin was not observed. This result suggested that nondialyzable soluble components were required for nuclear vesicle fusion. GTP gamma S and guanylyl imidodiphosphate significantly inhibited vesicle fusion but had no effect on vesicle binding to chromatin. Preincubation of membranes with 1 mM GTP gamma S or GTP did not impair vesicle binding or fusion when assayed with fresh cytosol. However, preincubation of membranes with GTP gamma S plus cytosol caused irreversible inhibition of fusion. The soluble factor mediating the inhibition by GTP gamma S, which we named GTP-dependent soluble factor (GSF), was titratable and was depleted from cytosol by incubation with excess membranes plus GTP gamma S, suggesting a stoichiometric interaction between GSF and a membrane component in the presence of GTP gamma S. In preliminary experiments, cytosol depleted of GSF remained active for fusion of chromatin-bound vesicles, suggesting that GSF may not be required for the fusion reaction itself. We propose that GTP hydrolysis is required at a step before the fusion of nuclear vesicles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.