ArticleDNA repair2007
The structure-specific nicking of small heteroduplexes by the RAG complex: implications for lymphoid chromosomal translocations.
Article in DNA repair, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- HIV integrase inhibitor, Elvitegravir, impairs RAG functions and inhibits V(D)J recombination.Cell death & disease · 2017Article
- PREVENTING THE CHROMOSOMAL TRANSLOCATIONS THAT CAUSE CANCER.Transactions of the American Clinical and Climatological Association · 2016Article
- Role of recombination activating genes in the generation of antigen receptor diversity and beyond.Immunology · 2012Review
- How does DNA break during chromosomal translocations?Nucleic acids research · 2011Review
- Formation of a G-quadruplex at the BCL2 major breakpoint region of the t(14;18) translocation in follicular lymphoma.Nucleic acids research · 2011Article
- Cytosines, but not purines, determine recombination activating gene (RAG)-induced breaks on heteroduplex DNA structures: implications for genomic instability.The Journal of biological chemistry · 2010Article
- Nonhomologous DNA end joining (NHEJ) and chromosomal translocations in humans.Sub-cellular biochemistry · 2010Review
- Gene conversion causing human inherited disease: evidence for involvement of non-B-DNA-forming sequences and recombination-promoting motifs in DNA breakage and repair.Human mutation · 2009Article
- Leaky severe combined immunodeficiency and aberrant DNA rearrangements due to a hypomorphic RAG1 mutation.Blood · 2009Article
- HMGB1/2 can target DNA for illegitimate cleavage by the RAG1/2 complex.BMC molecular biology · 2009Article
- Conformational variants of duplex DNA correlated with cytosine-rich chromosomal fragile sites.The Journal of biological chemistry · 2009Article
- Chromosomal location targets different MYC family gene members for oncogenic translocations.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Article
- Quantitative analyses of RAG-RSS interactions and conformations revealed by atomic force microscopy.Biochemistry · 2008Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
During V(D)J recombination, the RAG complex binds at recombination signal sequences and creates double-strand breaks. In addition to this sequence-specific recognition of the RSS, the RAG complex has been shown to be a structure-specific nuclease, cleaving 3' overhangs and 3' flaps, and, more recently, 10 nucleotides (nt) bubble (heteroduplex) structures. Here, we assess the smallest size heteroduplex that core and full-length RAGs can cleave. We also test whether bubbles adjacent to a partial RSS are nicked any differently or any more efficiently than bubbles that are surrounded by random sequence. These points are important in considering what types and what size of non-B DNA structure that the RAG complex can nick, and this helps assess the role of the RAG complex in mediating lymphoid chromosomal translocations. We find that the smallest bubble nicked by the RAG complex is 3nt, and proximity to a partial or full RSS sequence does not affect the nicking by RAGs. RAG nicking efficiency increases with the size of the heteroduplex and is only about two-fold less efficient than an RSS when the bubble is 6nt. We consider these findings in the context of RAG nicking at non-B DNA structures in lymphoid chromosomal translocations.
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