ArticleThe Journal of cell biology1992
Localization of the nucleolar protein NO38 in amphibian oocytes.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 91 citations in OpenAlex.
- Nucleocapsid Interacts with NPM1 and Protects it from Proteolytic Cleavage, Enhancing Cell Survival, and is Involved in PEDV Growth.Scientific reports · 2017Article
- Drosha protein levels are translationally regulated during Xenopus oocyte maturation.Molecular biology of the cell · 2014Article
- Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- NPM1/B23: A Multifunctional Chaperone in Ribosome Biogenesis and Chromatin Remodeling.Biochemistry research international · 2011Article
- The tripartite motif of nuclear factor 7 is required for its association with transcriptional units.Molecular and cellular biology · 2007Article
- Distinct in vivo roles for double-stranded RNA-binding domains of the Xenopus RNA-editing enzyme ADAR1 in chromosomal targeting.The Journal of cell biology · 2003Article
- An H/ACA guide RNA directs U2 pseudouridylation at two different sites in the branchpoint recognition region in Xenopus oocytes.RNA (New York, N.Y.) · 2002Article
- Xenopus LSm proteins bind U8 snoRNA via an internal evolutionarily conserved octamer sequence.Molecular and cellular biology · 2002Article
- The RNA binding activity of a ribosome biogenesis factor, nucleophosmin/B23, is modulated by phosphorylation with a cell cycle-dependent kinase and by association with its subtype.Molecular biology of the cell · 2002Article
- Conventional and nonconventional roles of the nucleolus.International review of cytology · 2002Review
- The snoRNA domain of vertebrate telomerase RNA functions to localize the RNA within the nucleus.RNA (New York, N.Y.) · 2001Article
- The human but not the Xenopus RNA-editing enzyme ADAR1 has an atypical nuclear localization signal and displays the characteristics of a shuttling protein.Molecular biology of the cell · 2001Article
- Cloning, expression and nuclear localization of human NPM3, a member of the nucleophosmin/nucleoplasmin family of nuclear chaperones.BMC genomics · 2001Article
- Novel properties of the protein kinase CK2-site-regulated nuclear- localization sequence of the interferon-induced nuclear factor IFI 16.The Biochemical journal · 2001Article
- Mutational analysis of fibrillarin and its mobility in living human cells.The Journal of cell biology · 2000Article
- Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.Molecular biology of the cell · 1999Article
- Coilin shuttles between the nucleus and cytoplasm in Xenopus oocytes.Molecular biology of the cell · 1999Article
- Localization in the nucleolus and coiled bodies of protein subunits of the ribonucleoprotein ribonuclease P.The Journal of cell biology · 1999Article
- The RNA-editing enzyme ADAR1 is localized to the nascent ribonucleoprotein matrix on Xenopus lampbrush chromosomes but specifically associates with an atypical loop.The Journal of cell biology · 1999Article
- Nuclear and nucleolar targeting of human ribosomal protein S25: common features shared with HIV-1 regulatory proteins.Oncogene · 1999Article
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2 authors at 1 institution in 1 country.
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Abstract
To examine the role of primary amino acid sequence in the localization of proteins within the nucleus, we studied the nucleolar protein NO38 of amphibian oocytes. We synthesized NO38 transcripts in vitro, injected them into the oocyte cytoplasm, and followed the distribution of the translation products. The injected RNA contained a short sequence encoding an epitope derived from the human c-myc protein. We used an mAb against this epitope to detect translation products from injected RNAs by Western blots and by immunofluoresent staining of cytological preparations. When full-length transcripts of NO38 were injected into oocytes, the translation products accumulated efficiently in the germinal vesicle, and a major fraction was localized in the multiple nucleoli. To identify protein domains involved in this nucleolus-specific accumulation, we prepared a series of carboxy-terminal deletions of the cDNA. Oocytes injected with RNA encoding truncated forms of NO38 were examined for altered patterns of protein accumulation. We defined a domain of about 24 amino acids near the carboxy terminus that was essential for nucleolar localization of NO38. This domain is separated by more than 70 amino acids from two putative nuclear localization signals near the middle of the molecule. Hybrid constructs were made which encoded part of Escherichia coli beta-galactosidase or pyruvate kinase fused to a long segment of NO38 containing the essential domain. Injection of RNA from these constructs showed that the essential domain was not sufficient to target the hybrid proteins to the nucleolus. We suggest that nucleolar accumulation of NO38 requires more than a single linear domain.
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