ArticleMolecular and biochemical parasitology2007
Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana.
Article in Molecular and biochemical parasitology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Identification of transporters essential for survival of Leishmania promastigotes in the digestive tract of sand flies.PLoS pathogens · 2026Article
- Unraveling Leishmania major Metacyclogenesis: A Comprehensive Analysis of Transcriptomic and Metabolomic ProfilesIranian biomedical journal · 2025Article
- Repurposing of known drugs for leishmaniasis treatment using bioinformatic predictions, in vitro validations and pharmacokinetic simulations.Journal of computer-aided molecular design · 2019Article
- Cellular landmarks of Trypanosoma brucei and Leishmania mexicana.Molecular and biochemical parasitology · 2019Article
- The crystal structure of glucokinase from Leishmania braziliensis.Molecular and biochemical parasitology · 2019Article
- Glucose Transporters and Virulence inmSphere · 2018Article
- Leishmania mexicana can utilize amino acids as major carbon sources in macrophages but not in animal models.Molecular microbiology · 2018Article
- G-Quadruplex Identification in the Genome of Protozoan Parasites Points to Naphthalene Diimide Ligands as New Antiparasitic Agents.Journal of medicinal chemistry · 2018Article
- Leishmania major UDP-sugar pyrophosphorylase salvages galactose for glycoconjugate biosynthesis.International journal for parasitology · 2015Article
- Flagellar membrane proteins in kinetoplastid parasites.IUBMB life · 2015Review
- Regulation and biological function of a flagellar glucose transporter in Leishmania mexicana: a potential glucose sensor.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Leishmania carbon metabolism in the macrophage phagolysosome- feast or famine?F1000Research · 2015Review
- Gluconeogenesis in Leishmania mexicana: contribution of glycerol kinase, phosphoenolpyruvate carboxykinase, and pyruvate phosphate dikinase.The Journal of biological chemistry · 2014Article
- The effect of tunicamycin on the glucose uptake, growth, and cellular adhesion in the protozoan parasite Crithidia fasciculata.Current microbiology · 2014Article
- Transport proteins of parasitic protists and their role in nutrient salvage.Frontiers in plant science · 2014Review
- 'Transient' genetic suppression facilitates generation of hexose transporter null mutants in Leishmania mexicana.Molecular microbiology · 2013Article
- Role of cytosolic glyceraldehyde-3-phosphate dehydrogenase in visceral organ infection by Leishmania donovani.Eukaryotic cell · 2013Article
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- Plasmodial sugar transporters as anti-malarial drug targets and comparisons with other protozoa.Malaria journal · 2011Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Glucose is a major source of energy and carbon in promastigotes of Leishmania mexicana, and its uptake is mediated by three glucose transporters whose genes are encoded within a single cluster. A null mutant in which the glucose transporter gene cluster was deleted by homologous gene replacement was generated previously and shown to grow more slowly than wild type promastigotes but not to be viable as amastigotes in primary tissue culture macrophages or in axenic culture. Further phenotypic characterization demonstrates that the null mutant is unable to import glucose, mannose, fructose, or galactose and that each of the three glucose transporter isoforms, LmGT1, LmGT2, and LmGT3, is capable of transporting each of these hexoses. Complementation of the null mutant with each isoform is able to restore growth in each of the four hexoses to wild type levels. Null mutant promastigotes are reduced in size to about 2/3 the volume of wild type parasites. In addition, the null mutants are significantly more sensitive to oxidative stress than their wild type counterparts. These results underscore the importance of glucose transporters in the parasite life cycle and suggest reasons for their non-viability in the disease-causing amastigote stage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.