ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association1992
Morphologic differentiation of colon carcinoma cell lines HT-29 and HT-29KM in rotating-wall vessels.
Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Gene-environmental influence of space and microgravity on red blood cells with sickle cell disease.NPJ genomic medicine · 2024Review
- Scaffold-based 3D cell culture models in cancer research.Journal of biomedical science · 2024Review
- 3D cell culture model: From ground experiment to microgravity study.Frontiers in bioengineering and biotechnology · 2023Review
- Key aspects for conception and construction of co-culture models of tumor-stroma interactions.Frontiers in bioengineering and biotechnology · 2023Review
- The Fight against Cancer by Microgravity: The Multicellular Spheroid as a Metastasis Model.International journal of molecular sciences · 2022Review
- The Fast Track for Intestinal Tumor Cell Differentiation and In Vitro Intestinal Models by Inorganic Topographic Surfaces.Pharmaceutics · 2022Article
- Current approaches for the regeneration and reconstruction of ocular surface in dry eye.Frontiers in medicine · 2022Review
- Update on the effects of microgravity on the musculoskeletal system.NPJ microgravity · 2021Review
- A Simulated Microgravity Environment Causes a Sustained Defect in Epithelial Barrier Function.Scientific reports · 2019Article
- Functional impairment triggered by altertoxin II (ATXII) in intestinal cells in vitro: cross-talk between cytotoxicity and mechanotransduction.Archives of toxicology · 2018Article
- Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age.Infection and immunity · 2018Review
- The third dimension: new developments in cell culture models for colorectal research.Cellular and molecular life sciences : CMLS · 2016Review
- Microgravity as a biological tool to examine host-pathogen interactions and to guide development of therapeutics and preventatives that target pathogenic bacteria.Pathogens and disease · 2016Review
- Three-dimensional cell culture models for investigating human viruses.Virologica Sinica · 2016Review
- Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity.Journal of visualized experiments : JoVE · 2016Article
- Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations.PloS one · 2016Article
- 3D tissue-like assemblies: A novel approach to investigate virus-cell interactions.Methods (San Diego, Calif.) · 2015Article
- Growing tissues in real and simulated microgravity: new methods for tissue engineering.Tissue engineering. Part B, Reviews · 2014Review
- Three-dimensional in vitro tumor models for cancer research and drug evaluation.Biotechnology advances · 2014Review
- The impact of simulated and real microgravity on bone cells and mesenchymal stem cells.BioMed research international · 2014Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new low shear stress microcarrier culture system has been developed at NASA's Johnson Space Center that permits three-dimensional tissue culture. Two established human colon adenocarcinoma cell lines, HT-29, an undifferentiated, and HT-29KM, a stable, moderately differentiated subline of HT-29, were grown in new tissue culture bioreactors called Rotating-Wall Vessels (RWVs). RWVs are used in conjunction with multicellular cocultivation to develop a unique in vitro tissue modeling system. Cells were cultivated on Cytodex-3 microcarrier beads, with and without mixed normal human colonic fibroblasts, which served as the mesenchymal layer. Culture of the tumor lines in the absence of fibroblasts produced spheroidlike growth and minimal differentiation. In contrast, when tumor lines were co-cultivated with normal colonic fibroblasts, initial growth was confined to the fibroblast population until the microcarriers were covered. The tumor cells then commenced proliferation at an accelerated rate, organizing themselves into three-dimensional tissue masses that achieved 1.0- to 1.5-cm diameters. The masses displayed glandular structures, apical and internal glandular microvilli, tight intercellular junctions, desmosomes, cellular polarity, sinusoid development, internalized mucin, and structural organization akin to normal colon crypt development. Differentiated samples were subjected to transmission and scanning electron microscopy and histologic analysis, revealing embryoniclike mesenchymal cells lining the areas around the growth matrices. Necrosis was minimal throughout the tissue masses. These data suggest that the RWV affords a new model for investigation and isolation of growth, regulatory, and structural processes within neoplastic and normal tissue.
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