ArticlePLoS pathogens2007
Characterization of a structural intermediate of flavivirus membrane fusion.
Article in PLoS pathogens, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- A Modular Design of a Cholera Toxin B Subunit-Scaffolded Sub-Virion Nanoparticle Vaccine Against West Nile Virus.Microbial biotechnology · 2026Article
- The structure of inactivated mature tick-borne encephalitis virus at 3.0 Å resolution.Emerging microbes & infections · 2024Article
- Computational design of prefusion-stabilized Herpesvirus gB trimers.bioRxiv : the preprint server for biology · 2024Article
- Review
- Recent advances in the study of zika virus structure, drug targets, and inhibitors.Frontiers in pharmacology · 2024Review
- Identifying citrus limonoids as a potential fusion inhibitor of DENV-2 virus through its in silico study and FTIR analysis.In silico pharmacology · 2024Article
- Impact of structural dynamics on biological functions of flaviviruses.The FEBS journal · 2023Review
- Review
- Larvicidal and histopathological efficacy of cinnamic acid analogues: a novel strategy to reduce the dengue vector competence.RSC advances · 2022Article
- Viral Membrane Fusion Proteins and RNA Sorting Mechanisms for the Molecular Delivery by Exosomes.Cells · 2021Review
- Extensive flavivirus E trimer breathing accompanies stem zippering of the post-fusion hairpin.EMBO reports · 2020Article
- Review
- Convalescent patient-derived monoclonal antibodies targeting different epitopes of E protein confer protection against Zika virus in a neonatal mouse model.Emerging microbes & infections · 2019Article
- Early Events in Japanese Encephalitis Virus Infection: Viral Entry.Pathogens (Basel, Switzerland) · 2018Review
- Tick-Borne Encephalitis Virus: A Structural View.Viruses · 2018Review
- Monomeric Intermediates Formed by Vesiculovirus Glycoprotein during Its Low-pH-induced Structural Transition.Journal of molecular biology · 2018Review
- In silico prediction of monovalent and chimeric tetravalent vaccines for prevention and treatment of dengue fever.Journal of biomedical research · 2017Article
- A novel mechanism of antibody-mediated enhancement of flavivirus infection.PLoS pathogens · 2017Article
- Method for measuring the unbinding energy of strongly-bound membrane-associated proteins.Biochimica et biophysica acta · 2016Article
- A humanized monoclonal antibody neutralizes yellow fever virus strain 17D-204 in vitro but does not protect a mouse model from disease.Antiviral research · 2016Article
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Authors and funding
5 authors.
Funding
Abstract
Viral membrane fusion proceeds through a sequence of steps that are driven by triggered conformational changes of viral envelope glycoproteins, so-called fusion proteins. Although high-resolution structural snapshots of viral fusion proteins in their prefusion and postfusion conformations are available, it has been difficult to define intermediate structures of the fusion pathway because of their transient nature. Flaviviruses possess a class II viral fusion protein (E) mediating fusion at acidic pH that is converted from a dimer to a trimer with a hairpin-like structure during the fusion process. Here we show for tick-borne encephalitis virus that exposure of virions to alkaline instead of acidic pH traps the particles in an intermediate conformation in which the E dimers dissociate and interact with target membranes via the fusion peptide without proceeding to the merger of the membranes. Further treatment to low pH, however, leads to fusion, suggesting that these monomers correspond to an as-yet-elusive intermediate required to convert the prefusion dimer into the postfusion trimer. Thus, the use of nonphysiological conditions allows a dissection of the flavivirus fusion process and the identification of two separate steps, in which membrane insertion of multiple copies of E monomers precedes the formation of hairpin-like trimers. This sequence of events provides important new insights for understanding the dynamic process of viral membrane fusion.
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