Evidence map›Paper›PMID 17302794›Full record

ArticleAnimal genetics2007

Construction of bovine whole-genome radiation hybrid and linkage maps using high-throughput genotyping.

S D McKay, R D Schnabel, B M Murdoch, J Aerts, C A Gill, C Gao, C Li, L K Matukumalli, P Stothard, Z Wang and 4 more

Abstract readComparative Study
In one paragraph

Article in Animal genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

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  19. A critical analysis of disease-associated DNA polymorphisms in the genes of cattle, goat, sheep, and pig.Mammalian genome : official journal of the International Mammalian Genome Society · 2008
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

S D McKayDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada. sdmckay@ualberta.ca
R D Schnabel
B M Murdoch
J Aerts
C A Gill
C Gao
C Li
L K Matukumalli
P Stothard
Z Wang
C P Van Tassell
J L Williams
J F Taylor
S S Moore

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-density whole-genome maps are essential for ordering genes or markers and aid in the assembly of genome sequence. To increase the density of markers on the bovine radiation hybrid map, and hence contribute to the assembly of the bovine genome sequence, an Illumina BeadStation was used to simultaneously type large numbers of markers on the Roslin-Cambridge 3000 rad bovine-hamster whole-genome radiation hybrid panel (WGRH3000). In five multiplex reactions, 6738 sequence tagged site (STS) markers were successfully typed on the WGRH3000 panel DNA. These STSs harboured SNPs that were developed as a result of the bovine genome sequencing initiative. Typically, the most time consuming and expensive part of creating high-density radiation hybrid (RH) maps is genotyping the markers on the RH panel with conventional approaches. Using the method described in this article, we have developed a high-density whole-genome RH map with 4690 loci and a linkage map with 2701 loci, with direct comparison to the bovine whole-genome sequence assembly (Btau_2.0) in a fraction of the time it would have taken with conventional typing and genotyping methods.

Indexed as

AnimalsCattleChromosome MappingGenetic MarkersGenomeGenotypeRadiation Hybrid MappingSequence Tagged SitesGenetic Markers

Identifiers

PMID17302794
PMCPMC2063635

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.